Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

被引：47

作者：

Zdanov, Stephanie

Debacq-Chainiaux, Florence

Remacle, Jose

Toussaint, Olivier

机构：

[1] Univ Namur FUNDP, Res Unit Cellular Biol URBC, B-5000 Namur, Belgium

[2] Eppendorf Array Technol, B-5000 Namur, Belgium

来源：

FEBS LETTERS | 2006年 / 580卷 / 27期

关键词：

senescence; oxidative stress; fibroblasts; telomerase; p38(MAPK); gene expression;

D O I：

10.1016/j.febslet.2006.10.064

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38(MAPK) was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38(MAPK) activity attenuated the increase in the proportion of cells positive for senescence associated P-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38(MAPK) inhibitor and p38(MAPK) small interferent RNA, we identified several p38(MAPK)-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：6455 / 6463

页数：9

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