Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2

被引：240

作者：

Li, Wenlin ^{[1
]}

Zhou, Hongyan ^{[1
]}

Abujarour, Ramzey ^{[1
]}

Zhu, Saiyong ^{[1
]}

Joo, Jin Young ^{[2
]}

Lin, Tongxiang ^{[1
]}

Hao, Ergeng ^{[3
]}

Schoeler, Hans R. ^{[2
]}

Hayek, Alberto ^{[3
]}

Ding, Sheng ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, Munster, Germany

[3] Univ Calif San Diego, Dept Pediat, Whittier Inst, La Jolla, CA 92093 USA

来源：

STEM CELLS | 2009年 / 27卷 / 12期

关键词：

Induced pluripotency; iPS; Pluripotent stem cells; Cell culture; HUMAN FIBROBLASTS; INDUCTION; METHYLATION; ACTIVATION; DERIVATION; EFFICIENT; OCT4;

D O I：

10.1002/stem.240

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named tranylcypromine), an inhibitor of lysine-specific demethylase 1, CHIR99021 can cause the reprogramming of human primary keratinocyte transduced with the two factors, Oct4 and Klf4. To our knowledge, this is the first time that human iPS cells have been generated from somatic cells without exogenous Sox2 expression. Our studies suggest that the GSK-3 inhibitor might have a general application to replace transcription factors in both mouse and human reprogramming. STEM CELLS 2009;27:2992-3000

引用

页码：2992 / 3000

页数：9

共 31 条

[31] Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins [J].

Zhou, Hongyan ;

Wu, Shili ;

Joo, Jin Young ;

Zhu, Saiyong ;

Han, Dong Wook ;

Lin, Tongxiang ;

Trauger, Sunia ;

Bien, Geoffery ;

Yao, Susan ;

Zhu, Yong ;

Siuzdak, Gary ;

Schoeler, Hans R. ;

Duan, Lingxun ;

Ding, Sheng .

CELL STEM CELL, 2009, 4 (05) :381-384

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