Evaluation of a Polyurethane Platform for Delivery of Nanohydroxyapatite and Decellularized Bone Particles in a Porous Three-Dimensional Scaffold

被引：16

作者：

Bow, Austin ^{[1
]}

Newby, Steven ^{[1
]}

Rifkin, Rebecca ^{[1
]}

Jackson, Bailey K. ^{[2
]}

Matavosian, Alicia ^{[1
]}

Griffin, Christopher ^{[2
]}

King, William ^{[2
]}

Alghazali, Karrer ^{[2
]}

Mhannawee, Anwer ^{[2
]}

Berryhill, Stuart B. ^{[3
]}

Morello, Roy ^{[3
]}

Hecht, Silke ^{[1
]}

Biris, Alexandru S. ^{[2
]}

Anderson, David E. ^{[1
]}

Bourdo, Shawn E. ^{[2
]}

Dhar, Madhu ^{[1
]}

机构：

[1] Univ Tennessee, Coll Vet Med, 2407 River Dr, Knoxville, TN 37996 USA

[2] Univ Arkansas, Ctr Integrat Nanotechnol Sci, 2801 South Univ Ave, Little Rock, AR 72204 USA

[3] Univ Arkansas Med Sci, Little Rock, AR 72205 USA

来源：

ACS APPLIED BIO MATERIALS | 2019年 / 2卷 / 05期

基金：

美国国家科学基金会;

关键词：

bone; regenerative; nanohydroxyapatite; nanocomposite; polyurethane; rat; in vivo; osteogenesis;

D O I：

10.1021/acsabm.8b00670

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

The complex dynamic nature of bone tissue presents a unique challenge for developing optimal biomaterials within the field of bone tissue engineering. Materials based on biological and physiological characteristics of natural bone have shown promise for inducing and promoting effective bone repair. Design of multicomposite scaffolds that incorporate both malleable and hard mineral components allows for intricate structures with nano- and macrosized mineral components to provide architectural elements that promote osteogenesis. The examined S-1 and S-2 scaffolds are multilayered constructs which differ only in the compositional ratio of nanohydroxyapatite (nHA) and decellularized bone particles (DBPs). The constructs incorporated previously studied nHA/polyurethane films interspersed with macrosized bone DBPs to stimulate integration with native tissue and induce osteogenic activity. In vitro assessment of cytocompatibility and osteostimulatory characteristics indicated that the scaffolds did not negatively impact cell health and demonstrated osteogenic effects. When the constructs were implanted in vivo, in a rat tibial defect model, the biocompatibility and osteogenic impact were confirmed. Material-treated defects were observed to not induce negative tissue reactions and, in those treated with S-1 scaffolds, exhibited greater levels of new bone formation. These results indicate that, while both scaffold designs were biocompatible, S-1 constructs demonstrate more effective biologically relevant nano-/macromineral architectural elements.

引用

页码：1815 / 1829

页数：15

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