Anti-inflammatory effect of coumarins isolated from Corydalis heterocarpa in HT-29 human colon carcinoma cells

被引：70

作者：

Kang, Kyong-Hwa ^{[1
]}

Kong, Chang-Suk ^{[2
]}

Seo, Youngwan ^{[3
]}

Kim, Moon-Moo ^{[4
]}

Kim, Se-Kwon ^{[1
,2
]}

机构：

[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

[2] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea

[3] Korea Maritime Univ, Div Marine Environm & Biosci, Pusan 606791, South Korea

[4] Dong Eui Univ, Dept Chem, Pusan 614010, South Korea

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2009年 / 47卷 / 08期

关键词：

Corydalis heterocarpa; Anti-inflammatory; HT-29; cells; Coumarin; NF-kappa B; NITRIC-OXIDE SYNTHASE; I-KAPPA-B; NECROSIS-FACTOR-ALPHA; COX-2; CYCLOOXYGENASE-2; INFLAMMATION; EXPRESSION; INDUCTION; INHIBITION; ALKALOIDS;

D O I：

10.1016/j.fct.2009.05.036

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

We investigated anti-inflammatory effects of two coumarins, columbianetin (A) and libanoridin (B), isolated from Corydalis heterocarpa in lipopolysaccharide (LPS)-stimulated HT-29 human colon carcinoma cells. Treatment with compound B inhibited the protein expression levels of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) in a dose-dependent manner in LPS-stimulated HT-29 cells, but compound A did not. Also, compound B had a higher inhibitory effect on production of cytokines such as IL-1 beta and TNF-alpha in LPS-stimulated HT-29 human colon carcinoma cells than those of compound A. Furthermore, we confirmed that LPS-induced transcription activity of NF-kappa B was inhibited by compound B. As a result of this study, compound B can be considered as a potential anti-inflammatory agent. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2129 / 2134

页数：6

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