High-purity magnesium interference screws promote fibrocartilaginous entheses regeneration in the anterior cruciate ligament reconstruction rabbit model via accumulation of BMP-2 and VEGF

被引:160
作者
Cheng, Pengfei [1 ]
Han, Pei [1 ]
Zhao, Changli [2 ]
Zhang, Shaoxiang [2 ,3 ]
Wu, Hongliu [2 ]
Ni, Jiahua [2 ]
Hou, Peng [1 ]
Zhang, Yuanzhuang [3 ]
Liu, Jingyi [3 ]
Xu, Haidong [3 ]
Liu, Shen [1 ]
Zhang, Xiaonong [2 ,3 ]
Zheng, Yufeng [4 ]
Chai, Yimin [1 ]
机构
[1] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthopaed, Shanghai 200233, Peoples R China
[2] Shanghai Jiao Tong Univ, State Key Lab Met Matrix Composites, Sch Mat Sci & Engn, Shanghai 200240, Peoples R China
[3] Suzhou Origin Med Technol Co Ltd, Suzhou 215513, Peoples R China
[4] Peking Univ, Dept Mat Sci & Engn, Coll Engn, Beijing 100871, Peoples R China
关键词
High-purity magnesium; Interference screw; Anterior cruciate ligament reconstruction; Fibrocartilaginous entheses; BMP-2; VEGF; ENDOTHELIAL GROWTH-FACTOR; TO-BONE INTERFACE; RAT MODEL; IN-VITRO; STROMAL CELLS; TENDON GRAFT; TUNNEL; IMPLANTATION; DEGRADATION; EXPRESSION;
D O I
10.1016/j.biomaterials.2015.12.005
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Interference screw in the fixation of autologous tendon graft to the bone tunnel is widely accepted for the reconstruction of anterior cruciate ligament (ACL), but the regeneration of fibrocartilaginous entheses could hardly be achieved with the traditional interference screw. In the present work, biodegradable high-purity magnesium (HP Mg) showed good cytocompatibility and promoted the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF), fibrocartilage markers (Aggrecan, COL2A1 and SOX-9), and glycosaminoglycan (GAG) production in vitro. The HP Mg screw was applied to fix the semitendinosus autograft to the femoral tunnel in a rabbit model of ACL reconstruction with titanium (Ti) screw as the control. The femur-tendon graft-tibia complex was retrieved at 3, 6, 9 and 12 weeks. Gross observation and range of motion (ROM) of the animal model reached normal levels at 12 weeks. No sign of host reaction was found in the X-ray scanning. The HP Mg group was comparable to the Ti group with respect to biomechanical properties of the reconstructed ACL, and the ultimate load to failure and stiffness increased 12 weeks after surgery. In the histological analysis, the HP Mg group formed distinct fibrocartilage transition zones at the tendon-bone interface 12 weeks after surgery, whereas a disorganized fibrocartilage layer was found in the Ti group. In the immunohistochemical analysis, highly positive staining of BMP-2, VEGF and the specific receptor for BMP-2 (BMPRIA) was shown at the tendon-bone interface of the HP Mg group compared with the Ti group. Furthermore, the HP Mg group had significantly higher expression of BMP-2 and VEGF than the Ti group in the early phase of tendon-bone healing, followed by enhanced expression of fibrocartilage markers and GAG production. Therefore we proposed that the stimulation of BMP-2 and VEGF by Mg ions was responsible for the fibrochondrogenesis of Mg materials. HP Mg was promising as a biodegradable interference screw with the potential to promote fibrocartilaginous entheses regeneration in ACL reconstruction. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:14 / 26
页数:13
相关论文
共 48 条
[1]
The in vivo and in vitro corrosion of high-purity magnesium and magnesium alloys WZ21 and AZ91 [J].
Abidin, Nor Ishida Zainal ;
Rolfe, Barbara ;
Owen, Helen ;
Malisano, Julian ;
Martin, Darren ;
Hofstetter, Joelle ;
Uggowitzer, Peter J. ;
Atrens, Andrej .
CORROSION SCIENCE, 2013, 75 :354-366
[2]
Reconstruction of the anterior cruciate ligament in females: A comparison of hamstring versus patellar tendon autograft [J].
Barrett, GR ;
Noojin, FK ;
Hartzog, CW ;
Nash, CR .
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY, 2002, 18 (01) :46-54
[3]
Intraarticular migration of a broken biodegradable interference screw after anterior cruciate ligament reconstruction [J].
Baums, M. H. ;
Zelle, B. A. ;
Schultz, W. ;
Ernstberger, T. ;
Klinger, H. -M. .
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 2006, 14 (09) :865-868
[4]
The effect of matrix metalloproteinase inhibition on tendon-to-bone healing in a rotator cuff repair model [J].
Bedi, Asheesh ;
Kovacevic, David ;
Hettrich, Carolyn ;
Gulotta, Lawrence V. ;
Ehteshami, John R. ;
Warren, Russell F. ;
Rodeo, Scott A. .
JOURNAL OF SHOULDER AND ELBOW SURGERY, 2010, 19 (03) :384-391
[5]
Quadrupled semitendinosus-gracilis autograft fixation in the femoral tunnel: A comparison between a metal and a bioabsorbable interference screw [J].
Caborn, DNM ;
Coen, M ;
Neef, R ;
Hamilton, D ;
Nyland, J ;
Johnson, DL .
ARTHROSCOPY, 1998, 14 (03) :241-245
[6]
Chang Chih-Hsiang, 2012, Biomed J, V35, P473, DOI 10.4103/2319-4170.104412
[7]
COOPER RR, 1970, J BONE JOINT SURG AM, VA 52, P1
[8]
Effects of fluctuant magnesium concentration on phenotype of the primary chondrocytes [J].
Dou, Yana ;
Li, Nan ;
Zheng, Yufeng ;
Ge, Zigang .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2014, 102 (12) :4455-4463
[9]
Biomechanical testing and degradation analysis of MgCa0.8 alloy screws: A comparative in vivo study in rabbits [J].
Erdmann, Nina ;
Angrisani, Nina ;
Reifenrath, Janin ;
Lucas, Arne ;
Thorey, Fritz ;
Bormann, Dirk ;
Meyer-Lindenberg, Andrea .
ACTA BIOMATERIALIA, 2011, 7 (03) :1421-1428
[10]
Revolutionizing orthopaedic biomaterials: The potential of biodegradable and bioresorbable magnesium-based materials for functional tissue engineering [J].
Farraro, Kathryn E. ;
Kim, Kwang E. ;
Woo, Savio L-Y. ;
Flowers, Jonquil R. ;
McCullough, Matthew B. .
JOURNAL OF BIOMECHANICS, 2014, 47 (09) :1979-1986