Genomic organization of the human and mouse stau genes

被引:6
作者
Brizard, F [1 ]
Luo, M [1 ]
DesGroseillers, L [1 ]
机构
[1] Univ Montreal, Dept Biochem, Montreal, PQ H3C 3J7, Canada
关键词
D O I
10.1089/10445490050043308
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mammalian Staufen is a double-stranded RNA-binding protein potentially involved in mRNA transport and localization. Recently, we reported that the human gene is located on chromosome 20, region q13.1. We now report the genomic organization of both the human and mouse stau genes. Amplification of genomic DNA and sequencing of the resulting PCR products indicated that the human and mouse genes are fragmented into 15 and 12 exons, distributed over at least 65 and 17 kb of genomic DNA, respectively. The three additional exons found in the human gene are subjected to differential splicing, generating four different transcripts. Corresponding exons have not been found in mouse transcripts. Apart from those three exons, the overall organization of the stau gene is similar in the two species, and the positions of the exon-intron junctions are perfectly conserved. Even an alternative choice between two splicing acceptor sites, which causes an insertion of 18 nucleotides in exon 5, is conserved in both humans and mice. An extremely G+C-rich region lacking canonical TATA and CAAT boxes was found upstream of the most 5' RACE sequence, suggesting that a housekeeping-like promoter drives the broad expression of Staufen in mammalian cells. This work represents the first step toward production of knockout mice and the elucidation of putative Staufen-linked hereditary diseases in humans.
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页码:331 / 339
页数:9
相关论文
共 26 条
[1]   MOLECULAR ANALYSIS OF CHROMOSOME 20Q DELETIONS ASSOCIATED WITH MYELOPROLIFERATIVE DISORDERS AND MYELODYSPLASTIC SYNDROMES [J].
ASIMAKOPOULOS, FA ;
WHITE, NJ ;
NACHEVA, E ;
GREEN, AR .
BLOOD, 1994, 84 (09) :3086-3094
[2]  
Beck C, 1994, Neurobiol Dis, V1, P95, DOI 10.1006/nbdi.1994.0012
[3]   Staufen-dependent localization of prospero mRNA contributes to neuroblast daughter-cell fate [J].
Broadus, J ;
Fuerstenberg, S ;
Doe, CQ .
NATURE, 1998, 391 (6669) :792-795
[4]   CONSERVED STRUCTURES AND DIVERSITY OF FUNCTIONS OF RNA-BINDING PROTEINS [J].
BURD, CG ;
DREYFUSS, G .
SCIENCE, 1994, 265 (5172) :615-621
[5]   NMR SOLUTION STRUCTURE OF A DSRNA BINDING DOMAIN FROM DROSOPHILA STAUFEN PROTEIN REVEALS HOMOLOGY TO THE N-TERMINAL DOMAIN OF RIBOSOMAL-PROTEIN S5 [J].
BYCROFT, M ;
GRUNERT, S ;
MURZIN, AG ;
PROCTOR, M ;
STJOHNSTON, D .
EMBO JOURNAL, 1995, 14 (14) :3563-3571
[6]   Localization of a human double-stranded RNA-binding protein gene (STAU) to band 20q13.1 by fluorescence in situ hybridization [J].
DesGroseillers, L ;
Lemieux, N .
GENOMICS, 1996, 36 (03) :527-529
[7]   IDENTIFICATION OF DOUBLE-STRANDED RNA-BINDING DOMAINS IN THE INTERFERON-INDUCED DOUBLE-STRANDED RNA-ACTIVATED P68 KINASE [J].
FENG, GS ;
CHONG, K ;
KUMAR, A ;
WILLIAMS, BRG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5447-5451
[8]   CHARACTERIZATION OF A HUMAN TAR RNA-BINDING PROTEIN THAT ACTIVATES THE HIV-1 LTR [J].
GATIGNOL, A ;
BUCKLERWHITE, A ;
BERKHOUT, B ;
JEANG, KT .
SCIENCE, 1991, 251 (5001) :1597-1600
[9]  
Hillier L, 1991, PCR Methods Appl, V1, P124
[10]   Molecular insights into mRNA transport and local translation in the mammalian nervous system [J].
Kiebler, MA ;
DesGroseillers, L .
NEURON, 2000, 25 (01) :19-28