The biological routes of gene delivery mediated by lipid-based non-viral vectors

被引：40

作者：

Duan, Yan ^{[1
,2
,3
]}

Zhang, Shubiao ^{[1
]}

Wang, Bing ^{[1
]}

Yang, Baoling ^{[1
]}

Zhi, Defu ^{[1
]}

机构：

[1] Dalian Nationalities Univ, Coll Life Sci, SEAC ME Key Lab Biotechnol & Bioresources Utiliza, Dalian 116600, Liaoning, Peoples R China

[2] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

[3] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

EXPERT OPINION ON DRUG DELIVERY | 2009年 / 6卷 / 12期

基金：

中国国家自然科学基金;

关键词：

gene delivery; non-viral vectors; technology; trafficking; CLATHRIN-INDEPENDENT ENDOCYTOSIS; MAMMALIAN-CELLS OCCURS; IN-VITRO; CATIONIC LIPOSOMES; PHYSICOCHEMICAL CHARACTERIZATION; ELECTRON-MICROSCOPY; DNA TRANSFECTION; COMPLEXES; PEPTIDE; SERUM;

D O I：

10.1517/17425240903287153

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Cationic lipid/DNA complexes (lipoplexes) represent an attractive alternative transfection in vitro and in vivo but still suffer from relatively low efficiency. Comprehension of the interactions between vectors and DNA as well as cellular pathways and mechanisms in DNA entry into cells and ultimately nuclei will lead to the design of better adapted non-viral vectors for gene therapy applications. Here, some recent developments in the field on the pathways and mechanisms involved in lipoplex-mediated transfection are discussed. The techniques that are widely used to study the mechanism of gene delivery are also discussed.

引用

页码：1351 / 1361

页数：11

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