Aptamers and riboswitches: perspectives in biotechnology

被引：49

作者：

Weigand, Julia E. ^{[1
]}

Suess, Beatrix ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Mol Biowissensch, Aventis Fdn Endowed Professorship, D-60438 Frankfurt, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2009年 / 85卷 / 02期

关键词：

RNA; Aptamer; Riboswitch; Conditional gene expression; Biosensor; IN-VITRO SELECTION; GENE-EXPRESSION; SYNTHETIC RIBOSWITCH; NATURAL RIBOSWITCH; MAMMALIAN-CELLS; RNA; BINDING; TRANSLATION; RECOGNITION; FLUOROPHORE;

D O I：

10.1007/s00253-009-2194-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aptamers are short, single stranded nucleic acids which bind a wide range of different ligands with extraordinary high binding affinity and specificity. The steadily increasing number of aptamers is accompanied by an expanding range of applications in biotechnology. We will describe new developments in the field including the use of aptamers for conditional gene regulation and as biosensors. In addition, we will discuss the potential of aptamers as tags to visualize RNA and protein distribution in living cells and as therapeutics. Furthermore, we will consider biotechnological applications of riboswitches for gene regulation and as drug target.

引用

页码：229 / 236

页数：8

共 73 条

[71] Semi-automated selection of DNA aptamers using magnetic particle handling [J].