Light activation as a method of regulating and studying gene expression

被引：105

作者：

Deiters, Alexander ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2009年 / 13卷 / 5-6期

基金：

美国国家卫生研究院;

关键词：

RNA INTERFERENCE; MAMMALIAN-CELLS; IN-VITRO; PROTEIN; KNOCKDOWN; ANTISENSE; ACIDS;

D O I：

10.1016/j.cbpa.2009.09.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, several advances have been made in the activation and deactivation of gene expression using light. These developments are based on the application of small molecule inducers of gene expression, antisense- or RNA interference-mediated gene silencing, and the photochemical control of proteins regulating gene function. The majority of the examples employ a classical 'caging technology', through the chemical installation of a light-removable protecting group on the biological molecule (small molecule, oligonucleotide, or protein) of interest and rendering it inactive. UV light irradiation then removes the caging group and activates the molecule, enabling control over gene activity with high spatial and temporal resolution.

引用

页码：678 / 686

页数：9

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