CDKB1;1 Forms a Functional Complex with CYCA2;3 to Suppress Endocycle Onset

被引：169

作者：

Boudolf, Veronique ^{[1
,2
]}

Lammens, Tim ^{[1
,2
]}

Boruc, Joanna ^{[1
,2
]}

Van Leene, Jelle ^{[1
,2
]}

Van Den Daele, Hilde ^{[1
,2
]}

Maes, Sara ^{[1
,2
]}

Van Isterdael, Gert ^{[1
,2
]}

Russinova, Eugenia ^{[1
,2
]}

Kondorosi, Eva ^{[3
,4
]}

Witters, Erwin ^{[5
,6
]}

De Jaeger, Geert ^{[1
,2
]}

Inze, Dirk ^{[1
,2
]}

De Veylder, Lieven ^{[1
,2
]}

机构：

[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium

[3] CNRS, Inst Sci Vegetal, Unite Propre Rech 2355, F-91198 Gif Sur Yvette, France

[4] Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, Hungary

[5] Univ Antwerp, Ctr Proteome Anal & Mass Spectrometry, Dept Biol, B-2020 Antwerp, Belgium

[6] Flemish Inst Technol Res, B-2400 Mol, Belgium

来源：

PLANT PHYSIOLOGY | 2009年 / 150卷 / 03期

关键词：

ANAPHASE-PROMOTING COMPLEX; CYCLIN-DEPENDENT-KINASE; CELL-CYCLE; ARABIDOPSIS-THALIANA; TRANSCRIPTION FACTOR; FISSION YEAST; S-PHASE; PLANT DEVELOPMENT; DESTRUCTION BOX; ENDOREDUPLICATION;

D O I：

10.1104/pp.109.140269

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.

引用

页码：1482 / 1493

页数：12

共 60 条

[51] Interplay between Arabidopsis activating factors E2Fb and E2Fa in cell cycle progression and development [J].