Recent insights on RNA folding mechanisms from catalytic RNA

被引:111
作者
Woodson, SA [1 ]
机构
[1] Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA
关键词
RNA folding; ribozyme; energy landscape; translation attenuation; RNA structure; group I intron; RNase P; folding kinetics; transcription elongation;
D O I
10.1007/s000180050042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly. These kinetically trapped intermediates arise from alternative secondary structures that form early in the folding process. In cells, proteins modulate the outcome of RNA folding reactions by stabilizing specific conformations or by accelerating refolding of misfolded intermediates. At the same time, competition between metastable conformations provides a means for regulating the biological activity of transcripts.
引用
收藏
页码:796 / 808
页数:13
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