Enzymatic Incorporation of Multiple Dyes for Increased Sensitivity in QD-FRET Sensing for DNA Methylation Detection

被引：27

作者：

Bailey, Vasudev J. ^{[1
]}

Keeley, Brian P. ^{[2
]}

Zhang, Yi ^{[1
]}

Ho, Yi-Ping ^{[2
]}

Easwaran, Hariharan ^{[3
]}

Brock, Malcolm V. ^{[3
]}

Pelosky, Kristen L. ^{[3
]}

Carraway, Hetty E. ^{[3
]}

Baylin, Stephen B. ^{[3
]}

Herman, James G. ^{[3
]}

Wang, Tza-Huei ^{[1
,2
]}

机构：

[1] Johns Hopkins Sch Med, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Baltimore, MD 21218 USA

[3] Johns Hopkins Sch Med, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 01期

基金：

美国国家科学基金会;

关键词：

biosensors; DNA; methylation; optical analysis; spectroscopic methods; CANCER; NANOSENSOR; ASSAY; PCR;

D O I：

10.1002/cbic.200900630

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) Come closer to the light: Fluorescently labeled cytosines have been enzymatically incorporated within bisulfite-treated DNA that was amplified by using methylation-specific primers. Detection of these multilabeled products was carried out by using quantum-dot FRET (QD-FRET). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：71 / 74

页数：4

共 20 条

[1]

[Anonymous], 1996, EPIGENETIC MECH GENE

[2] MS-qFRET: A quantum dot-based method for analysis of DNA methylation [J].