Micro-chemical imaging of cesium distribution in Arabidopsis thaliana plant and its interaction with potassium and essential trace elements

被引:70
作者
Isaure, M. -P.
Fraysse, A.
Deves, G.
Le Lay, P.
Fayard, B.
Susini, J.
Bourguignon, J.
Ortega, R.
机构
[1] Univ Bordeaux 1, CNRS, CNAB, Grp Imagerie Chim Cellulaire & Speciat,UMR 5084, F-33175 Gradignan, France
[2] CEA, DRT, LITEN, Sect Applicat Traceurs, F-38054 Grenoble 09, France
[3] UJF, UMR 5168, CNRS, CEA,DRDC,Inra,DSV,Lab Physiol Cellulaire Vegetale, F-38054 Grenoble 09, France
[4] European Synchrotron Radiat Facil, F-38043 Grenoble, France
关键词
cesium; potassium; trace element; chemical imaging; Arabidopsis thaliana;
D O I
10.1016/j.biochi.2006.08.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cesium as an alkali element exhibits a chemical reactivity similar to that of potassium, an essential element for plants. It has been suggested that Cs phytotoxicity might be due either to its competition with potassium to enter the plant, resulting in K starvation, or to its intracellular competition with K binding sites in cells. Such elemental interactions can be evidenced by chemical imaging, which determines the elemental distributions. In this study, the model plant Arabidopsis thaliana was exposed to 1 mM cesium in the presence (20 mM) or not of potassium. The quantitative imaging of Cs and endogenous elements (P, S, Cl, K, Ca, Mn, Fe, and Zn) was carried out using ion beam micro-chemical imaging with 5 mu m spatial resolution. Chemical imaging was also evidenced by microfocused synchrotron-based X-ray fluorescence (pXRF) which presents la better lateral resolution (< 1 mu m) but is not quantitative. Cesium distribution was similar to potassium which suggests that Cs can compete with K binding sites in cells. Cesium and potassium were mainly concentrated in the vascular system of stems and leaves. Cs was also found in lower concentration in leaves mesophyll/epidermis. This late representing the larger proportion in mass, mesophyll/epidermis can be considered as the major storage site for cesium in A. thaliana. Trichomes were not found to accumulate cesium. Interestingly, increased Mn, Fe, and Zn concentrations were observed in leaves at high chlorosis. Mn and Fe increased more in the mesophyll than in veins, whereas zinc increased more in veins than in the mesophyll suggesting a tissue specific interaction of Cs with these trace elements homeostasis. This study illustrates the sensitivity of ion beam microprobe and microfocused synchrotron-based X-ray fluorescence to investigate concentrations and distributions of major and trace elements in plants. It also shows the suitability of these analytical imaging techniques to complement biochemical investigations of metallic stress in plants. (c) 2006 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:1583 / 1590
页数:8
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