Temporal dynamics of brain tissue nitric oxide during functional forepaw stimulation in rats

被引：81

作者：

Buerk, DG ^{[1
]}

Ances, BM

Greenberg, JH

Detre, JA

机构：

[1] Univ Penn, Sch Med, Inst Environm Med, Dept Physiol,Dept Bioengn, Philadelphia, PA 19104 USA

[2] Univ Penn, Sch Med, Cerebrovasc Res Ctr, Dept Neurol, Philadelphia, PA 19104 USA

来源：

NEUROIMAGE | 2003年 / 18卷 / 01期

关键词：

cerebral blood flow; functional activation; laser-Doppler flowmetry; nitric oxide; nitric oxide synthase; nitric oxide microelectrode;

D O I：

10.1006/nimg.2002.1314

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We report the first dynamic measurements of tissue nitric oxide (NO) during functional activation of rat somatosensory cortex by electrical forepaw stimulation. Cortical tissue NO was measured electrochemically with rapid-responding recessed microelectrodes (tips < 10 mum). Simultaneous blood flow measurements were made by laser-Doppler flowmetry (LDF). NO immediately increased, reaching a peak 125.5 +/- 32.8 (SE) nM above baseline (P < 0.05) within 400 ms after stimulus onset, preceding any LDF changes, and then returned close to prestimulus levels after 2 s (123 signal-averaged trials, 12 rats). Blood flow began rising after a 1-s delay, reaching a peak just before electrical stimulation was ended at t = 4 s. A consistent poststimulus NO undershoot was observed as LDF returned to baseline. These findings complement our previous study (B. M. Ances et aL, 2001, Neurosci. Lett. 306,106-110) in which a transient decrease in rat somatosensory cortex tissue oxygen partial pressure was found to precede blood flow increases during functional activation. (C) 2002 Elsevier Science (USA).

引用

页码：1 / 9

页数：9

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