Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization

被引：111

作者：

Gerdts, Cory J.

Tereshko, Valentina

Yadav, Maneesh K.

Dementieva, Irina

Collart, Frank

Joachimiak, Andrzej

Stevens, Raymond C.

Kuhn, Peter

Kossiakoff, Anthony

Ismagilov, Rustem F.

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60615 USA

[2] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60615 USA

[3] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60615 USA

[4] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[5] Scripps Res Inst, Dept Cellular Biol, La Jolla, CA 92037 USA

[6] Univ Chicago, Pritzker Sch Med, Dept Pediat, Inst Mol Pediat Sci, Chicago, IL 60615 USA

[7] Argonne Natl Lab, Midw Ctr Struct Genom, Argonne, IL 60439 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 48期

关键词：

crystal growth; microfluidics; nucleation; protein structures;

D O I：

10.1002/anie.200602946

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sowing seeds: The growth of well-ordered protein crystals requires control of the two key stages of crystallization, namely, nucleation and growth. However, the ideal conditions for these two stages are often different. A microfluidic system (see picture) was used to perform time-controlled crystal seeding in nL volumes. Single crystals of the protein Oligoendopeptidase F were obtained and used to determine its X-ray crystal structure. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8156 / 8160

页数：5

共 29 条

[11] A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion [J].