A new generation of cross-linkers for selective detection by MALDI MS

被引：15

作者：

Paramelle, David ^{[1
,2
]}

Cantel, Sonia ^{[1
,2
]}

Enjalbal, Christine ^{[1
,2
]}

Amblard, Muriel ^{[1
,2
]}

Forest, Eric ^{[3
]}

Heymann, Michael ^{[4
]}

Geourjon, Christophe ^{[4
]}

Martinez, Jean ^{[1
,2
]}

Subra, Gilles ^{[1
,2
]}

机构：

[1] Univ Montpellier 1, CNRS, IBMM, UMR 5247, F-34000 Montpellier, France

[2] Univ Montpellier 2, CNRS, IBMM, UMR 5247, F-34000 Montpellier, France

[3] UJF, CNRS, CEA, Inst Biol Struct,Prot Mass Spectrometry Lab,UMR 5, Grenoble, France

[4] Univ Lyon 1, CNRS, Inst Biol & Chim Prot, Lab Bioinformat & RMN Struct,UMR 5086, F-69365 Lyon, France

来源：

PROTEOMICS | 2009年 / 9卷 / 23期

关键词：

Cross-linker; Matrix-assisted laser desorption; Protein structure prediction; Signal enhancement; Technology; UV-absorbing label; PROTEIN-PROTEIN INTERACTIONS; MASS-SPECTROMETRY; LINKING; PEPTIDES; REAGENTS; ESTERS; TOOL;

D O I：

10.1002/pmic.200900562

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We designed a new cross-linker bearing a CHCA moiety. The use of the CHCA-tagged crosslinker JMV 3378 in conjunction with a neutral MALDI matrix alpha-cyano-4-hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI-TOF MS of cross-link containing peptides. Discrimination between modified and non-modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the alpha-cyano-4-hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome G and apo-myoglobine as model proteins.

引用

页码：5384 / 5388

页数：5

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