Whisker-mediated transformation of embryogenic callus of maize

被引：33

作者：

Petolino, JF ^{[1
]}

Hopkins, NL ^{[1
]}

Kosegi, BD ^{[1
]}

Skokut, M ^{[1
]}

机构：

[1] Dow AgroSci LLC, Plant Transformat & Gene Express, Indianapolis, IN 46268 USA

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 08期

关键词：

silicon carbide whiskers; gene transfer; Type II maize callus;

D O I：

10.1007/s002999900180

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The present study was designed to establish embryogenic callus as a target tissue for whisker-mediated transformation of maize (Zea mays L.). Silicon carbide whiskers were used to deliver the bar and uidA (GUS) genes into embryogenic maize callus. Samples of osmotically-treated Type II callus were vigorously agitated in the presence of whiskers and plasmid DNA using a standard laboratory vortex or a modified dental amalgamator. On average, three transgenic callus lines were obtained per 100 samples treated. Plants were regenerated from several GUS-expressing callus lines and DNA analyses confirmed stable integration and inheritance. As with other direct DNA delivery methods involving embryogenic maize callus, integration patterns of the inserted DNA appeared to be complex. Although currently less efficient than microparticle bombardment on a per target basis, whisker-mediated transformation of embryogenic callus represents a viable method for transgenic maize production.

引用

页码：781 / 786

页数：6

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