Influence of chelators and iron ions on the production and degradation of H2O2 by β-amyloid-copper complexes

beta-Amyloid peptide (A beta) 1-42, involved in the pathogenesis of Alzheimer's disease, binds copper ions to form A beta (.) Cu-n complexes that are able to generate H2O2 in the presence of a reductant and O-2. The production of H2O2 can be stopped with chelators. More reactive than H2O2 itself, hydroxyl radicals HO. (generated when a reduced redox active metal complex interacts with H2O2) are also probably involved in the oxidative stress that creates brain damage during the disease. We report in the present work a method to monitor the effect of chelating agents on the production of hydrogen peroxide by metallo-amyloid peptides. The addition of H2O2 associated to a pre-incubation step between ascorbate and A beta (.) Cu-n allows to study the formation of H2O2 but also, at the same time, its transformation by the copper complexes. A beta (.) Cu-n peptides produce but do not efficiently degrade H2O2. The reported analytic method, associated to precipitation experiments of copper-containing amyloid peptides, allows to study the inhibition of H2O2 production by chelators. The action of a ligand such as EDTA is probably due to the removal of the copper ions from A beta (.) Cu-n, whereas bidentate ligands such as 8-hydroxyquinolines probably act via the formation of ternary complexes with A beta (.) Cu-n. The redox activity of these bidentate ligands can be modulated by the incorporation or the modification of substituents on the quinoline heterocycle. (c) 2006 Elsevier Inc. All rights reserved.