Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis

被引：38

作者：

Gordhan, BG

Andersen, SJ

DeMeyer, AR

Mizrahi, V

机构：

[1] S AFRICAN INST MED RES, MOL BIOL UNIT, ZA-2000 JOHANNESBURG, SOUTH AFRICA

[2] UNIV WITWATERSRAND, SCH MED, DEPT HEMATOL, ZA-2193 JOHANNESBURG, SOUTH AFRICA

来源：

GENE | 1996年 / 178卷 / 1-2期

关键词：

DNA polymerase I; DNA repair; Homologous recombination; Mycobacterium smegmatis; polA;

D O I：

10.1016/0378-1119(96)00350-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Gene replacement was achieved by homologous recombination in Mycobacterium smegmatis (Ms) using a cloned segment of the polA gene (encoding the DNA polymerase I) disrupted within the region encoding the C-terminal DNA polymerase domain by a kanamycin-resistance marker. The Ms polA755:aph mutant thus generated displayed a phenotype of hypersensitivity to DNA damage induced by UV irradiation and by hydrogen peroxide challenge.

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页码：125 / 130

页数：6

相关论文

共 27 条

[1] THE URAA LOCUS AND HOMOLOGOUS RECOMBINATION IN MYCOBACTERIUM-BOVIS BCG [J].

ALDOVINI, A ;

HUSSON, RN ;

YOUNG, RA .

JOURNAL OF BACTERIOLOGY, 1993, 175 (22) :7282-7289

[2] INHA, A GENE ENCODING A TARGET FOR ISONIAZID AND ETHIONAMIDE IN MYCOBACTERIUM-TUBERCULOSIS [J].

BANERJEE, A ;

DUBNAU, E ;

QUEMARD, A ;

BALASUBRAMANIAN, V ;

UM, KS ;

WILSON, T ;

COLLINS, D ;

DELISLE, G ;

JACOBS, WR .

SCIENCE, 1994, 263 (5144) :227-230

[3]

BEAMAN L, 1984, ANNU REV MICROBIOL, V38, P27

[4] RECOMBINATION-DEFICIENT MUTANTS OF SALMONELLA-TYPHIMURIUM ARE AVIRULENT AND SENSITIVE TO THE OXIDATIVE BURST OF MACROPHAGES [J].

BUCHMEIER, NA ;

LIPPS, CJ ;

SO, MYH ;

HEFFRON, F .

MOLECULAR MICROBIOLOGY, 1993, 7 (06) :933-936

[5]

CANETTI G, 1963, B WORLD HEALTH ORGAN, V29, P565

[6] DIRECT DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY DNA AMPLIFICATION [J].

DEWIT, D ;

STEYN, L ;

SHOEMAKER, S ;

SOGIN, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (11) :2437-2441

[7] THE 5' TO 3' EXONUCLEASE ACTIVITY OF DNA-POLYMERASE-I IS ESSENTIAL FOR STREPTOCOCCUS-PNEUMONIAE [J].

DIAZ, A ;

LACKS, SA ;

LOPEZ, P .

MOLECULAR MICROBIOLOGY, 1992, 6 (20) :3009-3019

[8] THE MYCOBACTERIUM-LEPRAE GENOME - SYSTEMATIC SEQUENCE-ANALYSIS IDENTIFIES KEY CATABOLIC ENZYMES, ATP-DEPENDENT TRANSPORT-SYSTEMS AND A NOVEL POLA LOCUS ASSOCIATED WITH GENOMIC VARIABILITY [J].

FSIHI, H ;

COLE, ST .

MOLECULAR MICROBIOLOGY, 1995, 16 (05) :909-919

[9] IDENTIFICATION, SEQUENCING, AND TARGETED MUTAGENESIS OF A DNA-POLYMERASE GENE REQUIRED FOR THE EXTREME RADIORESISTANCE OF DEINOCOCCUS-RADIODURANS [J].

GUTMAN, PD ;

FUCHS, P ;

LING, OY ;

MINTON, KW .

JOURNAL OF BACTERIOLOGY, 1993, 175 (11) :3581-3590

[10] CLONING AND SEQUENCE-ANALYSIS OF THE GENE ENCODING THE DNA POLYMERASE-I FROM MYCOBACTERIUM-TUBERCULOSIS [J].

HUBERTS, P ;

MIZRAHI, V .

GENE, 1995, 164 (01) :133-136