Enzymatic synthesis of sialylation substrates powered by a novel polyphosphate kinase (PPK3)

被引：77

作者：

Nahalka, Jozef ^{[1
]}

Patoprsty, Vladimir ^{[1
]}

机构：

[1] Slovak Acad Sci, Ctr Glyc, Inst Chem, SK-84538 Bratislava, Slovakia

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2009年 / 7卷 / 09期

关键词：

CMP-SIALIC ACID; ESCHERICHIA-COLI; RECOMBINANT; REGENERATION; PROTEINS; GLYCOPROTEINS; AGGREGATION; ENZYMES; CELLS; GENE;

D O I：

10.1039/b822549b

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Active inclusion bodies of polyphosphate kinase 3 and cytidine 5'-monophosphate kinase were combined with whole cells that co-express sialic acid aldolase and CMP-sialic acid synthetase. The biocatalytic mixture was used for the synthesis of CMP-sialic acid, which was then converted to 3'-sialyllactose by whole cells.

引用

页码：1778 / 1780

页数：3

共 38 条

[1]

AKIYAMA M, 1992, J BIOL CHEM, V267, P22556

[2] AN EFFICIENT SYNTHESIS OF CYTIDINE MONOPHOSPHO-SIALIC ACIDS WITH 4 IMMOBILIZED ENZYMES [J].

AUGE, C ;

GAUTHERON, C .

TETRAHEDRON LETTERS, 1988, 29 (07) :789-790

[3] Outstanding stability of immobilized recombinant α(1→3/4)-fucosyltransferases exploited in the synthesis of Lewis a and Lewis x trisaccharides [J].

Augé, C ;

Malleron, A ;

Tahrat, H ;

Marc, A ;

Goergen, JL ;

Cerutti, M ;

Steelant, WFA ;

Delannoy, P ;

Lubineau, A .

CHEMICAL COMMUNICATIONS, 2000, (20) :2017-2018

[4] Biosynthesis of human-type N-glycans in heterollogous systems [J].

Betenbaugh, MJ ;

Tomiya, N ;

Narang, S ;

Hsu, JTA ;

Lee, YC .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2004, 14 (05) :601-606

[5] Enhanced sialylation of EPO by overexpression of UDP-GlcNAc 2-epimerase/ManAc kinase containing a sialuria mutation in CHO cells [J].

Bork, Kaya ;

Reutter, Werner ;

Weldermann, Wenke ;

Horstkorte, Rudiger .

FEBS LETTERS, 2007, 581 (22) :4195-4198

[6] Galactosylation and sialylation of terminal glycan residues of human immunoglobulin G using bacterial glycosyltransferases with in situ regeneration of sugar-nucleotides [J].

Chung, Seung-Wook ;

Joo, Hwang-Soo ;

Jang, Kyoung-Soon ;

Lee, Hwa-Jin ;

Lee, Sun-Gu ;

Kim, Byung-Gee .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 39 (01) :60-66

[7]

Dingermann Theo, 2008, Biotechnology Journal, V3, P90, DOI 10.1002/biot.200700214

[8] Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins -: art. no. 27 [J].

García-Fruitós, E ;

González-Montalbán, N ;

Morell, M ;

Vera, A ;

Ferraz, RM ;

Arís, A ;

Ventura, S ;

Villaverde, A .

MICROBIAL CELL FACTORIES, 2005, 4 (1)

[9] Recombinant protein solubility - does more mean better? [J].

Gonzalez-Montalban, Nuria ;

Garcia-Fruitos, Elena ;

Villaverde, Antonio .

NATURE BIOTECHNOLOGY, 2007, 25 (07) :718-720

[10] The Ashwell receptor mitigates the lethal coagulopathy of sepsis [J].

Grewal, Prabhjit K. ;

Uchiyama, Satoshi ;

Ditto, David ;

Varki, Nissi ;

Le, Dzung T. ;

Nizet, Victor ;

Marth, Jamey D. .

NATURE MEDICINE, 2008, 14 (06) :648-655

← 1 2 3 4 →