Cell death in bovine parvovirus-infected embryonic bovine tracheal cells is mediated by necrosis rather than apoptosis

被引:38
作者
Abdel-Latif, Lubna [1 ]
Murray, Byron K. [1 ]
Renberg, Rebecca L. [1 ]
O'Neill, Kim L. [1 ]
Porter, Heidi [1 ]
Jensen, James B. [1 ]
Johnson, F. Brent [1 ]
机构
[1] Brigham Young Univ, Dept Microbiol & Mol Biol, Provo, UT 84602 USA
关键词
D O I
10.1099/vir.0.81915-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The helper-independent bovine parvovirus (BPV) was studied to determine its effect on host embryonic bovine tracheal (EBTr) cells: whether the ultimate outcome of infection results in apoptotic cell death or cell death by necrosis. Infected cells were observed for changes marking apoptosis. Observations of alterations in nuclear morphology, membrane changes, apoptotic body formation, membrane phosphatidylserine inversions, caspase activation and cell DNA laddering in infected cells were not indicative of apoptosis. On the other hand, at the end of the virus replication cycle, infected cells released viral haemagglutinin and infectious virus particles, as would be expected from cell membrane failure. Moreover, the infected cells released lactate dehydrogenase (LDH), release of which is a marker of necrosis. LDH release into the cell medium correlated directly with viral m.o.i. and time post-infection. Furthermore, assessment of mitochondrial dehydrogenase activity was consistent with cell death by necrosis. Taken together, these findings indicate that cell death in BPV-infected EBTr cells is due to necrosis, as defined by infected-cell membrane failure and release of the cell contents into the extracellular environment.
引用
收藏
页码:2539 / 2548
页数:10
相关论文
共 44 条
[11]  
CLARK C, 1999, BLOOD WKLY 1025, P7
[12]   STUDIES ON THE REPLICATION OF A BOVINE PARVOVIRUS [J].
DURHAM, PJK ;
JOHNSON, RH .
VETERINARY MICROBIOLOGY, 1985, 10 (02) :165-177
[13]   The NS2 proteins of parvovirus minute virus of mice are required for efficient nuclear egress of progeny virions in mouse cells [J].
Eichwald, V ;
Daeffler, L ;
Klein, M ;
Rommelaere, J ;
Salomé, N .
JOURNAL OF VIROLOGY, 2002, 76 (20) :10307-10319
[14]  
FAUQUET CM, 2005, VIRUS TAXONOMY 8 REP, P353
[15]   Vesicular stomatitis viruses expressing wild-type or mutant M proteins activate apoptosis through distinct pathways [J].
Gaddy, DF ;
Lyles, DS .
JOURNAL OF VIROLOGY, 2005, 79 (07) :4170-4179
[16]   The biochemistry of apoptosis [J].
Hengartner, MO .
NATURE, 2000, 407 (6805) :770-776
[17]   A RAPID AND SIMPLE METHOD FOR THE ISOLATION OF APOPTOTIC DNA FRAGMENTS [J].
HERRMANN, M ;
LORENZ, HM ;
VOLL, R ;
GRUNKE, M ;
WOITH, W ;
KALDEN, JR .
NUCLEIC ACIDS RESEARCH, 1994, 22 (24) :5506-5507
[18]   Human parvovirus B19 non-structural protein (NS1) induces apoptosis through mitochondria cell death pathway in COS-7 cells [J].
Hsu, TC ;
Wu, WJ ;
Chen, MC ;
Tsay, GJ .
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 2004, 36 (08) :570-577
[19]   Apoptosis in feline panleukopenia virus-infected lymphocytes [J].
Ikeda, Y ;
Shinozuka, J ;
Miyazawa, T ;
Kurosawa, K ;
Izumiya, Y ;
Nishimura, Y ;
Nakamura, K ;
Cai, JS ;
Fujita, K ;
Doi, K ;
Mikami, T .
JOURNAL OF VIROLOGY, 1998, 72 (08) :6932-6936
[20]   STRUCTURAL PROTEINS OF HADEN VIRUS [J].
JOHNSON, FB ;
HOGGAN, MD .
VIROLOGY, 1973, 51 (01) :129-137