Presence of a Na+/H+ exchanger in acidocalcisomes of Leishmania donovani and their alkalization by anti-leishmanial drugs

被引：50

作者：

Vercesi, AE

Rodrigues, CO

Catisti, R

Docampo, R

机构：

[1] Univ Illinois, Dept Pathobiol, Mol Parasitol Lab, Urbana, IL 61802 USA

[2] Univ Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, BR-13083970 Campinas, SP, Brazil

来源：

FEBS LETTERS | 2000年 / 473卷 / 02期

关键词：

acidocalcisome; Na+/H+ exchanger; 3,5-dibutyl-4-hydroxytoluene; volutin; Leishmania donovani;

D O I：

10.1016/S0014-5793(00)01531-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acidocalcisomes are acidic vacuoles present in trypanosomatids that contain most of the cellular calcium. The data presented here demonstrate that Leishmania donovani acidocalcisomes possess a Na+/H+ exchanger. 3,5-Dibutyl-4-hydroxytoulene, in the concentration range of 0-20 mu M, inhibited the Na+/H+ exchanger, and strongly stimulated the activity of the vacuolar H+-ATPase responsible for vacuolar acidification. As occurs with Na+, the cationic anti-leishmanial drugs pentamidine, WR-6026, and chloroquine promoted a fast and extensive alkalization of the L, donovani acidocalcisomes. (C) 2000 Federation of European Biochemical Societies.

引用

页码：203 / 206

页数：4

共 23 条

[1] Evidence for involvement of a zymogen granule Na+/H+ exchanger in enzyme secretion from rat pancreatic acinar cells [J].