Human colostrum and serum contain antibodies reactive to the intimin-binding region of the enteropathogenic Escherichia coli translocated intimin receptor

被引:27
作者
Sanches, MI
Keller, R
Hartland, EL
Figueiredo, DMM
Batchelor, M
Martinez, MB
Dougan, G
Careiro-Sampaio, MMS
Frankel, G [1 ]
Trabulsi, LR
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, England
[2] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Dept Immunol, Sao Paulo, Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo, Brazil
关键词
colostrum immunoglobulin A; enteropathogenic Escherichia coli; intimin; Tir;
D O I
10.1097/00005176-200001000-00021
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: In Brazil, enteropathogenic Escherichia coli (EPEC) diarrhoea is endemic in young infants. A characteristic feature of EPEC adhesion to host cells is intimate attachment leading to the formation of distinctive "attaching and effacing" (A/E) lesions on mammalian cells. Two genes directly involved in intimate adhesion, ene and tir, encode the adhesion molecule intimin and its translocated receptor Tir, respectively. The intimin-binding domain of Tir was recently mapped to the middle part of the polypeptide (Tir-M), and the amino (Tir-N) and carboxy (Tir-C) termini were found to be located within infected host cells. Recently, it was shown that colostrum samples from mothers living in Sao Paulo contain IgA-class antibodies reactive with a number of proteins associated with EPEC virulence. It has also been shown that patients infected with verocytotoxin-producing E. coli O157 can produce antibodies to Tir. In the current study antibody responses to the different Tir domains were analyzed in sera and colostrum samples collected in an EPEC-endemic area of Brazil. Methods: Recombinant Tir, Tir-N, Tir-M, and Tir-C were expressed as His-tagged protein in E. coli BL21a and purified on nickel columns. Western blot analysis was used to investigate colostrum IgA- and serum IgG-class antibodies reactive with the Tir fragments. Results: Anti-Tir IgG antibodies were detected in the serum of children, with (63%) or without (50%) diarrhoea, Anti-Tir IgA-class antibodies were detected in all the colostrum pools tested. With the use of both serum IgG- and colostrum IgA-class antibodies, an immunodominant domain of the Tir-polypeptide, Tir M, was identified. Conclusion: The intimin-binding region of Tir (Tir-M) is the immunodominant region of the polypeptide in humans. Both serum IgG-class and colostrum IgA-class antibodies reacted predominantly with the Tir-M domain. (C) 2000 Lippincott Williams & Wilkins, Inc.
引用
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页码:73 / 77
页数:5
相关论文
共 21 条
[11]   A GENETIC-LOCUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI NECESSARY FOR THE PRODUCTION OF ATTACHING AND EFFACING LESIONS ON TISSUE-CULTURE CELLS [J].
JERSE, AE ;
YU, J ;
TALL, BD ;
KAPER, JB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) :7839-7843
[12]  
Kelly G, 1999, NAT STRUCT BIOL, V6, P313
[13]   Enteropathogenic E-coli (EPEC) transfers its receptor for intimate adherence into mammalian cells [J].
Kenny, B ;
DeVinney, R ;
Stein, M ;
Reinscheid, DJ ;
Frey, EA ;
Finlay, BB .
CELL, 1997, 91 (04) :511-520
[14]   ENTEROPATHOGENIC ESCHERICHIA-COLI OF CLASSIC SEROTYPES ASSOCIATED WITH INFANT DIARRHEA - EPIDEMIOLOGY AND PATHOGENESIS [J].
LEVINE, MM ;
EDELMAN, R .
EPIDEMIOLOGIC REVIEWS, 1984, 6 :31-51
[15]  
Levine MM, 1978, LANCET, V1, P119
[16]   Human colostrum contains IgA antibodies reactive to enteropathogenic Escherichia coli virulence-associated proteins: Intimin, BfpA, EspA, and EspB [J].
Loureiro, I ;
Frankel, G ;
Adu-Bobie, J ;
Dougan, G ;
Trabulsi, LR ;
Carneiro-Sampaio, MMS .
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1998, 27 (02) :166-171
[17]   Antibody response of children with enteropathogenic Escherichia coli infection to the bundle-forming pilus and locus of enterocyte effacement-encoded virulence determinants [J].
Martinez, MB ;
Taddei, CR ;
Ruiz-Tagle, A ;
Trabulsi, LR ;
Girón, JA .
JOURNAL OF INFECTIOUS DISEASES, 1999, 179 (01) :269-274
[18]   A GENETIC-LOCUS OF ENTEROCYTE EFFACEMENT CONSERVED AMONG DIVERSE ENTEROBACTERIAL PATHOGENS [J].
MCDANIEL, TK ;
JARVIS, KG ;
DONNENBERG, MS ;
KAPER, JB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (05) :1664-1668
[19]  
NAGAO AT, 1999, IMMUNOL LETT, V96, P187
[20]   Translocated intimin receptors (Tir) of Shiga-toxigenic Escherichia coli isolates belonging to serogroups O26, O111, and O157 react with sera from patients with hemolytic-uremic syndrome and exhibit marked sequence heterogeneity [J].
Paton, AW ;
Manning, PA ;
Woodrow, MC ;
Paton, JC .
INFECTION AND IMMUNITY, 1998, 66 (11) :5580-5586