A PARACEST MRI contrast agent to detect enzyme activity

被引：154

作者：

Yoo, Byunghee ^{[1
]}

Pagel, Marty D. ^{[1
]}

机构：

[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 43期

关键词：

D O I：

10.1021/ja063874f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A "smart" PARACEST MRI contrast agent was synthesized to detect caspase-3, which is an important biomarker in apoptosis. The well-known caspase-3 substrate, DEVD (Asp-Glu-Val-Asp), was elongated using the amino group on one sidearm of lanthanide ligand anchored on the polymer support. The amide of DEVD-(Tm-DOTA) showed a PARACEST effect with MR saturation at -51 ppm. DEVD-(Tm-DOTA) amide was successfully cleaved by caspase-3, exposing the free amine group, which showed PARACEST with saturation at +8 ppm. The enzymatic activity of caspase-3 can be detected by the change in PARACEST effect caused by this biotransformation. Copyright © 2006 American Chemical Society.

引用

页码：14032 / 14033

页数：2

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