A PARACEST MRI contrast agent to detect enzyme activity

被引：154

作者：

Yoo, Byunghee ^{[1
]}

Pagel, Marty D. ^{[1
]}

机构：

[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 43期

关键词：

D O I：

10.1021/ja063874f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A "smart" PARACEST MRI contrast agent was synthesized to detect caspase-3, which is an important biomarker in apoptosis. The well-known caspase-3 substrate, DEVD (Asp-Glu-Val-Asp), was elongated using the amino group on one sidearm of lanthanide ligand anchored on the polymer support. The amide of DEVD-(Tm-DOTA) showed a PARACEST effect with MR saturation at -51 ppm. DEVD-(Tm-DOTA) amide was successfully cleaved by caspase-3, exposing the free amine group, which showed PARACEST with saturation at +8 ppm. The enzymatic activity of caspase-3 can be detected by the change in PARACEST effect caused by this biotransformation. Copyright © 2006 American Chemical Society.

引用

页码：14032 / 14033

页数：2

共 19 条

[11] IDENTIFICATION AND INHIBITION OF THE ICE/CED-3 PROTEASE NECESSARY FOR MAMMALIAN APOPTOSIS [J].

NICHOLSON, DW ;

ALI, A ;

THORNBERRY, NA ;

VAILLANCOURT, JP ;

DING, CK ;

GALLANT, M ;

GAREAU, Y ;

GRIFFIN, PR ;

LABELLE, M ;

LAZEBNIK, YA ;

MUNDAY, NA ;

RAJU, SM ;

SMULSON, ME ;

YAMIN, TT ;

YU, VL ;

MILLER, DK .

NATURE, 1995, 376 (6535) :37-43

[12] Magnetic relaxation switches capable of sensing molecular interactions [J].

Perez, JM ;

Josephson, L ;

O'Loughlin, T ;

Högemann, D ;

Weissleder, R .

NATURE BIOTECHNOLOGY, 2002, 20 (08) :816-820

[13] Selective sensing of zinc ions with a PARACEST contrast agent [J].

Trokowski, R ;

Ren, JM ;

Kálmán, FK ;

Sherry, AD .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (42) :6920-6923

[14] Cyclen-based phenylboronate ligands and their Eu3+ complexes for sensing glucose by MRI [J].

Trokowski, R ;

Zhang, SR ;

Sherry, AD .

BIOCONJUGATE CHEMISTRY, 2004, 15 (06) :1431-1440

[15] Paramagnetic lanthanide complexes as PARACEST agents for medical imaging [J].

Woods, Mark ;

Woessner, Donald E. ;

Sherry, A. Dean .

CHEMICAL SOCIETY REVIEWS, 2006, 35 (06) :500-511

[16] MRI thermometry based on PARACEST agents [J].

Zhang, SR ;

Malloy, CR ;

Sherry, AD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (50) :17572-17573

[17] A paramagnetic CEST agent for imaging glucose by MRI [J].

Zhang, SR ;

Trokowski, R ;

Sherry, AD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (50) :15288-15289

[18] PARACEST agents: Modulating MRI contrast via water proton exchange [J].

Zhang, SR ;

Merritt, M ;

Woessner, DE ;

Lenkinski, RE ;

Sherry, AD .

ACCOUNTS OF CHEMICAL RESEARCH, 2003, 36 (10) :783-790

[19] A novel europium(III)-based MRI contrast agent [J].

Zhang, SR ;

Winter, P ;

Wu, KC ;

Sherry, AD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (07) :1517-1518

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