A paramagnetic MRI-CEST agent responsive to lactate concentration

被引：144

作者：

Aime, S ^{[1
]}

Delli Castelli, D ^{[1
]}

Fedeli, F ^{[1
]}

Terreno, E ^{[1
]}

机构：

[1] IFM, Dipartimento Chim, I-10125 Turin, Italy

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 32期

关键词：

D O I：

10.1021/ja0264044

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

A paramagnetic Yb(III) complex bearing six exchangeable amide protons, [Yb(MBDO3AM)]3+, has been investigated with the aim of developing a MRI-CEST (chemical exchange saturation transfer) contrast agent responsive to the concentration of L-lactate. The complex binds the substrate quantitatively to yield [Yb(MBDO3AM)L-lactate]2+. The exchange between the free and the L-lactate-bound complex is slow on the NMR time scale, and the resonances of their corresponding amide protons are sufficiently separated (more than 10 ppm) to allow their selective irradiation. Therefore, the CEST properties of the two forms can be independently assessed. In turn, the resulting saturation transfer to the bulk water signal is dependent on the L-lactate concentration. Copyright © 2002 American Chemical Society.

引用

收藏

页码：9364 / 9365

页数：2

相关论文

共 15 条

[1]

Separation of intra- and extracellular lactate NMR signals using a lanthanide shift reagent [J].

Aime, S ;

Botta, M ;

Mainero, V ;

Terreno, E .

MAGNETIC RESONANCE IN MEDICINE, 2002, 47 (01) :10-13

[2]

Paramagnetic lanthanide(III) complexes as pH-sensitive chemical exchange saturation transfer (CEST) contrast agents for MRI applications [J].

Aime, S ;

Barge, A ;

Delli Castelli, D ;

Fedeli, F ;

Mortillaro, A ;

Nielsen, FU ;

Terreno, E .

MAGNETIC RESONANCE IN MEDICINE, 2002, 47 (04) :639-648

[3]

Modulation of the water exchange rates in [Gd-DO3A] complex by formation of ternary complexes with carboxylate ligands [J].

Aime, S ;

Botta, M ;

Bruce, JI ;

Mainero, V ;

Parker, D ;

Terreno, E .

CHEMICAL COMMUNICATIONS, 2001, (01) :115-116

[4]

The selectivity of reversible oxy-anion binding in aqueous solution at a chiral europium and terbium center: Signaling of carbonate chelation by changes in the form and circular polarization of luminescence emission [J].

Bruce, JI ;

Dickins, RS ;

Govenlock, LJ ;

Gunnlaugsson, T ;

Lopinski, S ;

Lowe, MP ;

Parker, D ;

Peacock, RD ;

Perry, JJB ;

Aime, S ;

Botta, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (40) :9674-9684

[5]

Dagher AP, 2000, J MAGN RESON IMAGING, V12, P745, DOI 10.1002/1522-2586(200011)12:5<745::AID-JMRI12>3.0.CO

[6]

2-H

[7]

Bidentate lactate binding in aqueous solution in a cationic, heptadentate lanthanide complex: an effective chiral derivatising agent [J].

Dickins, RS ;

Love, CS ;

Puschmann, H .

CHEMICAL COMMUNICATIONS, 2001, (22) :2308-2309

[8]

Sensitive NMR detection of cationic-polymer-based gene delivery systems using saturation transfer via proton exchange [J].

Goffeney, N ;

Bulte, JWM ;

Duyn, J ;

Bryant, LH ;

van Zijl, PCM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (35) :8628-8629

[9]

PETERS AR, 1996, PROG NMR SPECTROSC, V28, P283

[10]

Ward KM, 2000, MAGN RESON MED, V44, P799, DOI 10.1002/1522-2594(200011)44:5<799::AID-MRM18>3.0.CO