Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

被引：33

作者：

Jeon, Eugene ^{[1
]}

Hyeon, Jeong-eun ^{[1
]}

Suh, Dong Jin ^{[2
]}

Suh, Young-Woong ^{[2
]}

Kim, Seoung Wook ^{[3
]}

Song, Kwang Ho ^{[3
]}

Han, Sung Ok ^{[1
]}

机构：

[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Korea Inst Sci & Technol, Clean Energy Res Ctr, Seoul 136791, South Korea

[3] Korea Univ, Dept Chem Biol Engn, Seoul 136701, South Korea

来源：

MOLECULES AND CELLS | 2009年 / 28卷 / 04期

关键词：

beta-glucosidase; cellulose degradation; Clostridium thermocellum; endoglucanase; ethanol production; extracellular expression; Saccharomyces cerevisiae; RECOMBINANT KLEBSIELLA-OXYTOCA; AMORPHOUS CELLULOSE; MICROCRYSTALLINE CELLULOSE; CRYSTALLINE CELLULOSE; YEAST-STRAIN; FERMENTATION; DEGRADATION; HYDROLYSIS; CELLULASES; CELLOBIOSE;

D O I：

10.1007/s10059-009-0131-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and beta-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an alpha-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and beta-glucosidase was able to produce ethanol from beta-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

引用

页码：369 / 373

页数：5

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