Investigation of peak compression effects in packed column supercritical fluid chromatography using chemometrics

被引：10

作者：

Carlsson, D ^{[1
]}

Strode, JT ^{[1
]}

Gyllenhaal, O ^{[1
]}

Karlsson, A ^{[1
]}

Karlsson, L ^{[1
]}

机构：

[1] AB HASSLE,ANALYT CHEM,S-43183 MOLNDAL,SWEDEN

来源：

CHROMATOGRAPHIA | 1997年 / 44卷 / 5-6期

关键词：

supercritical fluid chromatography; peak compression; chemometrics;

D O I：

10.1007/BF02466397

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper chemometrics have been used to study and characterize peak compression phenomena in packed column SFC. A carbon dioxide/2-methyl-1-propanol mobile phase was used in the experimental design (modifier concentration, temperature and pressure) and modelling part of the investigation. A cubic interaction term was needed in the model to obtain a reasonable fit, suggesting that all three parameters are of significance in terms of controlling peak compression. At the optimum conditions derived from the model a narrow peak was obtained as predicted.

引用

收藏

页码：289 / 294

页数：6

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