ENO1 gene product binds to the c-myc promoter and acts as a transcriptional repressor:: relationship with Myc promoter-binding protein 1 (MBP-1)

The Myc promoter-binding protein-1 (MBP-1) is a 37-38 kDa protein that binds to the c-myc P2 promoter and negatively regulates transcription of the protooncogene, MBP-I cDNA shares 97% similarity with the cDNA encoding the glycolytic enzyme alpha-enolase and both genes have been mapped to the same region of human chromosome 1, suggesting the hypothesis that the two proteins might he encoded by the same gene. We shove here data indicating that a 37 kDa protein is alternatively translated from the full-length alpha-enolase mRNA, This shorter form of alpha-enolase is able to bind the MBP-1 consensus sequence and to downregulate expression of a luciferase reporter gene under the control of the c-myc P2 promoter. Furthermore, using alpha-enolase/green fluorescent protein chimeras in transfection experiments we show that, while the 48 kDa alpha-enolase mainly has a cytoplasmic localization, the 37 kDa alpha-enolase is preferentially localized in the cell nuclei, The finding that a transcriptional repressor of the c-myc oncogene is an alternatively translated product of the ENO1 gene, which maps to a region of human chromosome I frequently deleted in human cancers, makes ENO1 a potential candidate for tumor suppressor. (C) 2000 Federation of European Biochemical Societies.