Involvement of the xyloglucan endotransglycosylase/hydrolases encoded by celery XTH1 and Arabidopsis XTH33 in the phloem response to aphids

被引:69
作者
Divol, Fanchon
Vilaine, Francoise
Thibivilliers, Sandra
Kusiak, Chantal
Sauge, Marie Helene
Dinant, Sylvie [1 ]
机构
[1] INRA, Lab Biol Cellulaire, UR501, F-78026 Versailles, France
[2] CSIC, IRTA, Lab Genet Mol Vegetal, Barcelona 08034, Spain
[3] INRA, UR115, Plant & Syst Culture Hort, F-84914 Avignon, France
[4] Univ Missouri, Dept Microbiol & Plant Pathol, Columbia, MO 65211 USA
关键词
Apium graveolens; Arabidopsis thaliana; Myzus persicae; cell wall; compatible interaction; plant-aphid interaction;
D O I
10.1111/j.1365-3040.2006.01618.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
During infestation, phloem-feeding insects induce transcriptional reprogramming in plants that may lead to protection. Transcripts of the celery XTH1 gene, encoding a xyloglucan endotransglycosylase/hydrolase (XTH), were previously found to accumulate systemically in celery (Apium graveolens) phloem, following infestation with the generalist aphid Myzus persicae. XTH1 induction was specific to the phloem but was not correlated with an increase in xyloglucan endotransglycosylase (XET) activity in the phloem. XTH1 is homologous to the Arabidopsis thaliana XTH33 gene. XTH33 expression was investigated following M. persicae infestation. The pattern of XTH33 expression is tightly controlled during development and indicates a possible role in cell expansion. An xth33 mutant was assayed for preference assay with M. persicae. Aphids settled preferentially on the mutant rather than on the wild type. This suggests that XTH33 is involved in protecting plants against aphids; therefore, that cell wall modification can alter the preference of aphids for a particular plant. Nevertheless, the ectopic expression of XTH33 in phloem tissue was not sufficient to confer protection, demonstrating that modifying the expression of this single gene does not readily alter plant-aphid interactions.
引用
收藏
页码:187 / 201
页数:15
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