Transduction of hippocampal CA1 by adenovirus in vivo

被引：8

作者：

Kammesheidt, A

Ito, KI

Kato, K

Villarreal, LP

Sumikawa, K

机构：

[1] UNIV CALIF IRVINE, DEPT PSYCHOBIOL, IRVINE, CA 92697 USA

[2] UNIV CALIF IRVINE, DEPT MOL BIOL & BIOCHEM, IRVINE, CA 92697 USA

来源：

BRAIN RESEARCH | 1996年 / 736卷 / 1-2期

关键词：

adenovirus; hippocampus; rat; in vivo; long-term potentiation;

D O I：

10.1016/0006-8993(96)00715-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Replication-deficient adenoviral recombinants were assessed for in vivo transduction of rat hippocampal CA1 cells. Results show that efficient widespread transduction of CA1 in vivo was rapidly achievable and was sustained for more than 5 weeks. Assessment of electrophysiological properties in acute hippocampal slices showed that synaptic functioning and mechanisms involved in long-term potentiation (LTP) were preserved for minimally 5 weeks postinfection. Hence, adenovirus-mediated gene transfer in vivo promises to be a valuable tool for dissecting molecular mechanisms of synaptic plasticity, such as LTP and long-term depression (LTD).

引用

收藏

页码：297 / 304

页数：8

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