A catalog of human cDNA expression clones and its application to structural genomics -: art. no. R71

被引：15

作者：

Büssow, K

Quedenau, C

Sievert, V

Tischer, J

Scheich, C

Seitz, H

Hieke, B

Niesen, FH

Götz, F

Harttig, U

Lehrach, H

机构：

[1] Prot Struct Factory, D-14059 Berlin, Germany

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[3] Charite Univ Med Berlin, Inst Med Phys & Biophys, D-10117 Berlin, Germany

[4] Alpha Bioverfahrenstech GmbH, D-14532 Kleinmachnow, Germany

[5] Genome Res GmbH, RZPD German Resource Ctr, D-14059 Berlin, Germany

来源：

GENOME BIOLOGY | 2004年 / 5卷 / 09期

关键词：

D O I：

10.1186/gb-2004-5-9-r71

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

引用

页数：8

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[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].