Application of the yeast two-hybrid system in molecular gerontology

被引：8

作者：

Knudsen, CR ^{[1
]}

Jadidi, M ^{[1
]}

Friis, I ^{[1
]}

Mansilla, F ^{[1
]}

机构：

[1] Aarhus Univ, Inst Mol & Struct Biol, DK-8000 Aarhus C, Denmark

来源：

BIOGERONTOLOGY | 2002年 / 3卷 / 04期

关键词：

ageing; Alzheimer's disease; apoptosis; cellular senescence; protein-protein interactions; protein repair; telomere shortening; two-hybrid system; Werner's syndrome;

D O I：

10.1023/A:1016219121897

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Most - if not all - proteins are bound to interact with other proteins to exert their function, and thus the identification of the interaction partners of a protein is vital in proteomics. The yeast two-hybrid system is a popular and effective tool for studying protein-protein interactions. Although the advantages of the system are manifold, it also has certain drawbacks and limitations. The two-hybrid system has been shown to be extremely useful for placing a protein of unknown function within a functional context, thereby providing information about a putative role of the uncharacterised protein. This concept has also been successfully applied in molecular gerontology.

引用

页码：243 / 256

页数：14

共 64 条

[51] A two-hybrid dual bait system to discriminate specificity of protein interactions [J].

Serebriiskii, I ;

Khazak, V ;

Golemis, EA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (24) :17080-17087

[52] A positive genetic selection for disrupting protein-protein interactions: Identification of CREB mutations that prevent association with the coactivator CBP [J].

Shih, HM ;

Goldman, PS ;

DeMaggio, AJ ;

Hollenberg, SM ;

Goodman, RH ;

Hoekstra, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) :13896-13901

[53] CONSTRUCTION AND CHARACTERIZATION OF A NORMALIZED CDNA LIBRARY [J].

SOARES, MB ;

BONALDO, MD ;

JELENE, P ;

SU, L ;

LAWTON, L ;

EFSTRATIADIS, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9228-9232

[54] A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo [J].

Stagljar, I ;

Korostensky, C ;

Johnsson, N ;

te Heesen, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (09) :5187-5192

[55] Pex19p dampens the p19ARF-p53-p21WAF1 tumor suppressor pathway [J].

Sugihara, T ;

Kaul, SC ;

Kato, J ;

Reddel, RR ;

Nomura, H ;

Wadhwa, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (22) :18649-18652

[56] Werner protein recruits DNA polymerase δ to the nucleolus [J].

Szekely, AM ;

Chen, YH ;

Zhang, CY ;

Oshima, J ;

Weissman, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (21) :11365-11370

[57] X11L2, a new member of the X11 protein family, interacts with Alzheimer's β-amyloid precursor protein [J].

Tanahashi, H ;

Tabira, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 255 (03) :663-667

[58]

Tsan JT, 1997, ADV MOLEC BIOL, P217

[59] A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae [J].

Uetz, P ;

Giot, L ;

Cagney, G ;

Mansfield, TA ;

Judson, RS ;

Knight, JR ;

Lockshon, D ;

Narayan, V ;

Srinivasan, M ;

Pochart, P ;

Qureshi-Emili, A ;

Li, Y ;

Godwin, B ;

Conover, D ;

Kalbfleisch, T ;

Vijayadamodar, G ;

Yang, MJ ;

Johnston, M ;

Fields, S ;

Rothberg, JM .

NATURE, 2000, 403 (6770) :623-627

[60] Yeast forward and reverse 'n'-hybrid systems [J].

Vidal, M ;

Legrain, P .

NUCLEIC ACIDS RESEARCH, 1999, 27 (04) :919-929

← 1 2 3 4 5 6 7 →