Inhibition of DNA synthesis by K+-stabilised G-quadruplex promotes allelic preferential amplification

被引：21

作者：

Boán, F ^{[1
]}

Blanco, MG ^{[1
]}

Barros, P ^{[1
]}

González, AI ^{[1
]}

Gómez-Márquez, J ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Biol, Dept Bioquim & Biol Mol, Galicia, Spain

来源：

FEBS LETTERS | 2004年 / 571卷 / 1-3期

关键词：

minisatellite MsH43; K+-dependent G-quadruplex; stalling of DNA polymerization; preferential amplification;

D O I：

10.1016/j.febslet.2004.06.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR preferential amplification consists of the inefficient amplification of one allele in a heterozygous sample. Here, we report the isolation of a GC-rich human minisatellite, MsH43, that undergoes allelic preferential amplification during PCR. This effect requires the existence of a (TGGGGC)(4) motif that is able to form a G-quadruplex in the presence of K+. This structure interferes with the DNA synthesis of the alleles harbouring this motif during PCR The present results are the first demonstration that the formation of G-quadruplex can be one of the mechanisms involved in some kinds of preferential amplification. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：112 / 118

页数：7

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