Inhibition of DNA synthesis by K+-stabilised G-quadruplex promotes allelic preferential amplification

被引：21

作者：

Boán, F ^{[1
]}

Blanco, MG ^{[1
]}

Barros, P ^{[1
]}

González, AI ^{[1
]}

Gómez-Márquez, J ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Biol, Dept Bioquim & Biol Mol, Galicia, Spain

来源：

FEBS LETTERS | 2004年 / 571卷 / 1-3期

关键词：

minisatellite MsH43; K+-dependent G-quadruplex; stalling of DNA polymerization; preferential amplification;

D O I：

10.1016/j.febslet.2004.06.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR preferential amplification consists of the inefficient amplification of one allele in a heterozygous sample. Here, we report the isolation of a GC-rich human minisatellite, MsH43, that undergoes allelic preferential amplification during PCR. This effect requires the existence of a (TGGGGC)(4) motif that is able to form a G-quadruplex in the presence of K+. This structure interferes with the DNA synthesis of the alleles harbouring this motif during PCR The present results are the first demonstration that the formation of G-quadruplex can be one of the mechanisms involved in some kinds of preferential amplification. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：112 / 118

页数：7

共 38 条

[21]

Maxam A M, 1980, Methods Enzymol, V65, P499

[22] Crystal structure of parallel quadruplexes from human telomeric DNA [J].

Parkinson, GN ;

Lee, MPH ;

Neidle, S .

NATURE, 2002, 417 (6891) :876-880

[23] Human telomeric DNA: G-quadruplex, i-motif and watson-crick double helix [J].

Phan, AT ;

Mergny, JL .

NUCLEIC ACIDS RESEARCH, 2002, 30 (21) :4618-4625

[24] THE THERMODYNAMICS OF DNA STRUCTURES THAT CONTAIN LESIONS OR GUANINE TETRADS [J].

PILCH, DS ;

PLUM, GE ;

BRESLAUER, KJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (03) :334-342

[25] Stability of intramolecular DNA quadruplexes: Comparison with DNA duplexes [J].

Risitano, A ;

Fox, KR .

BIOCHEMISTRY, 2003, 42 (21) :6507-6513

[26] FORMATION OF PARALLEL 4-STRANDED COMPLEXES BY GUANINE-RICH MOTIFS IN DNA AND ITS IMPLICATIONS FOR MEIOSIS [J].

SEN, D ;

GILBERT, W .

NATURE, 1988, 334 (6180) :364-366

[27] Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c-MYC transcription [J].

Siddiqui-Jain, A ;

Grand, CL ;

Bearss, DJ ;

Hurley, LH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (18) :11593-11598

[28] G-quadruplex DNA structures - Variations on a theme [J].

Simonsson, T .

BIOLOGICAL CHEMISTRY, 2001, 382 (04) :621-628

[29] A human nuclease specific for G4 DNA [J].

Sun, H ;

Yabuki, A ;

Maizels, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (22) :12444-12449

[30] Minisateilites: mutability and genome architecture [J].

Vergnaud, G ;

Denoeud, F .

GENOME RESEARCH, 2000, 10 (07) :899-907

← 1 2 3 4 →