Recent advances in the biocatalytic reduction of ketones and oxidation of sec-alcohols

被引：350

作者：

Kroutil, W ^{[1
]}

Mang, H ^{[1
]}

Edegger, K ^{[1
]}

Faber, K ^{[1
]}

机构：

[1] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz, Austria

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2004年 / 8卷 / 02期

关键词：

D O I：

10.1016/j.cbpa.2004.02.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve the efficiency and applicability of biocatalytic redox-reactions for asymmetric ketone-reduction and enantioselective alcohol-oxidation catalyzed by nicotinamide-dependent dehydrogenases/reductases, several achievements for cofactor-recycling have been made during the last two years. First, the use of hydrogenases for NADPH recycling in a two enzyme system. Second, preparative transformations with alcohol dehydrogenases coupled with NADH oxidases for NAD(+)/NADP(+) recycling. Third, an exceptional chemo-stable alcohol dehydrogenase can efficiently use i-propanol and acetone as cosubstrates for reduction and oxidation, respectively, in a single-enzyme system. Novel carbonyl reductases and dehydrogenases derived from plant cells are particularly suited for sterically demanding substrates.

引用

页码：120 / 126

页数：7

共 52 条

[31] The crystal structure of R-specific alcohol dehydrogenase from Lactobacillus brevis suggests the structural basis of its metal dependency [J].