Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo

被引：454

作者：

North, TE

de Bruijin, MFTR

Stacy, T

Talebian, L

Lind, E

Robin, C

Binder, M

Dzierzak, E

Speck, NA ^{[1
]}

机构：

[1] Dartmouth Coll Sch Med, Dept Biochem, Hanover, NH 03755 USA

[2] Erasmus Univ, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands

来源：

IMMUNITY | 2002年 / 16卷 / 05期

关键词：

D O I：

10.1016/S1074-7613(02)00296-0

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1(+/-) embryos are heterogeneous and include CD45(+) cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of embryonic HSCs and contribute new insight into their cellular origin.

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收藏

页码：661 / 672

页数：12

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