Structural and functional characterization of the Bacillus megaterium uvrBA locus and generation of UV-sensitive mutants

被引：20

作者：

Nahrstedt, H ^{[1
]}

Meinhardt, F ^{[1
]}

机构：

[1] Univ Munster, Inst Mol Mikrobiol & Biotechnol, D-48149 Munster, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2004年 / 65卷 / 02期

关键词：

D O I：

10.1007/s00253-004-1572-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Bacillus megaterium genes uvrB and uvrA, encoding two subunits of the (A)BC excinuclease, which is responsible for nucleotide excision repair, were isolated and functionally characterized. RNA analyses revealed co-transcription of both genes probably forming a bicistronic operon. Expression of uvrB and uvrA was inducible by the DNA-damaging agent mitomycin C. This finding agrees with the presence of a potential DinR box within the uvrBA promoter. Single inactivation of uvrB or uvrA as well as the parallel knockout of both genes resulted in mutants highly sensitive to UV irradiation. Thus, this locus represents an attractive target for generating biologically safe containment strains of B. megaterium.

引用

页码：193 / 199

页数：7

共 40 条

[21]

NAHRSTEDT H, 2003, APPL MICROBIOL BIOTE

[22]

NAVARATNAM S, 1989, J BIOL CHEM, V264, P16067

[23] THE (A)BC EXCINUCLEASE OF ESCHERICHIA-COLI HAS ONLY THE UVRB AND UVRC SUBUNITS IN THE INCISION COMPLEX [J].

ORREN, DK ;

SANCAR, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5237-5241

[24] A NOVEL FORM OF TISSUE-SPECIFIC RNA PROCESSING PRODUCES APOLIPOPROTEIN-B48 IN INTESTINE [J].

POWELL, LM ;

WALLIS, SC ;

PEASE, RJ ;

EDWARDS, YH ;

KNOTT, TJ ;

SCOTT, J .

CELL, 1987, 50 (06) :831-840

[25]

Radman M, 1975, Basic Life Sci, V5A, P355

[26]

Sambrook J, 1989, MOL CLONING LAB MANU

[27] DNA excision repair [J].

Sancar, A .

ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :43-81

[28] The β-hairpin motif of UvrB is essential for DNA binding, damage processing, and UvrC-mediated incisions [J].

Skorvaga, M ;

Theis, K ;

Mandavilli, BS ;

Kisker, C ;

Van Houten, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (02) :1553-1559

[29] Localization of UvrA and effect of DNA damage on the chromosome of Bacillus subtilis [J].

Smith, BT ;

Grossman, AD ;

Walker, GC .

JOURNAL OF BACTERIOLOGY, 2002, 184 (02) :488-493

[30] Crystal structure of Escherichia coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction [J].

Sohi, M ;

Alexandrovich, A ;

Moolenaar, G ;

Visse, R ;

Goosen, N ;

Vernede, X ;

Fontecilla-Camps, JC ;

Champness, J ;

Sanderson, MR .

FEBS LETTERS, 2000, 465 (2-3) :161-164

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