Adenovirus E1A activates cyclin A gene transcription in the absence of growth factors through interaction with p107

被引:34
作者
Zerfass, K
Spitkovsky, D
Schulze, A
Joswig, S
Henglein, B
JansenDurr, P
机构
[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,FORSCH SCHWERPUNKT ANGEW TUMORVIROL,ABT 620,D-69120 HEIDELBERG,GERMANY
[2] INST CURIE,CNRS,F-75005 PARIS,FRANCE
关键词
D O I
10.1128/JVI.70.4.2637-2642.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using the infection of quiescent human fibroblasts with adenovirus type 5 and various deletion mutants, we show that E1A can stimulate transcription of the cyclin A gene in the absence of exogenous growth factors. Required for this activity is conserved region 2 (CR2), while both the N-terminal part of E1A and CR1 are dispensable. This indicates that activation of cyclin A gene expression requires the binding of E1A to p107, while binding to either pRB or p300 is not involved in transcriptional activation. We demonstrate that p107 represses the cyclin A promoter through its cell cycle-regulatory E2F binding site and that 12S E1A can activate the cyclin A promoter, essentially by counteracting its repression by p107. Since CR2 is required for cell transformation, transcriptional activation of the cyclin A gene by E1A appears to be important for its capacity to override control of cellular growth.
引用
收藏
页码:2637 / 2642
页数:6
相关论文
共 44 条
[21]   INTERACTION OF THE DR1 INHIBITORY FACTOR WITH THE TATA-BINDING PROTEIN IS DISRUPTED BY ADENOVIRUS-E1A [J].
KRAUS, VB ;
INOSTROZA, JA ;
YEUNG, K ;
REINBERG, D ;
NEVINS, JR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6279-6282
[22]   DRTF1/E2F - AN EXPANDING FAMILY OF HETERODIMERIC TRANSCRIPTION FACTORS IMPLICATED IN CELL-CYCLE CONTROL [J].
LATHANGUE, NB .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (03) :108-114
[23]   ADENOVIRUS E1A ACTIVATION DOMAIN BINDS THE BASIC REPEAT IN THE TATA BOX TRANSCRIPTION FACTOR [J].
LEE, WS ;
KAO, CC ;
BRYANT, GO ;
LIU, X ;
BERK, AJ .
CELL, 1991, 67 (02) :365-376
[24]   MULTIPLE FUNCTIONAL DOMAINS IN THE ADENOVIRUS E1A GENE [J].
MORAN, E ;
MATHEWS, MB .
CELL, 1987, 48 (02) :177-178
[25]  
MORO AM, UNPUB
[26]   TRANSCRIPTIONAL ACTIVATION BY THE ADENOVIRUS E1A PROTEINS [J].
NEVINS, JR .
SEMINARS IN VIROLOGY, 1993, 4 (01) :25-31
[27]   ASSOCIATION OF CDK2 KINASE WITH THE TRANSCRIPTION FACTOR-E2F DURING S-PHASE [J].
PAGANO, M ;
DRAETTA, G ;
JANSENDURR, P .
SCIENCE, 1992, 255 (5048) :1144-1147
[28]   CYCLIN-A IS REQUIRED AT 2 POINTS IN THE HUMAN CELL-CYCLE [J].
PAGANO, M ;
PEPPERKOK, R ;
VERDE, F ;
ANSORGE, W ;
DRAETTA, G .
EMBO JOURNAL, 1992, 11 (03) :961-971
[29]   THE ADENOVIRUS E1A PROTEINS INDUCE APOPTOSIS, WHICH IS INHIBITED BY THE E1B 19-KDA AND BCL-2 PROTEINS [J].
RAO, L ;
DEBBAS, M ;
SABBATINI, P ;
HOCKENBERY, D ;
KORSMEYER, S ;
WHITE, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7742-7746