Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection

被引:72
作者
Yang, Xuan [1 ,2 ]
Li, Tao [1 ,2 ]
Li, Bingling [1 ,2 ]
Wang, Erkang [1 ,2 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
基金
中国国家自然科学基金;
关键词
COLORIMETRIC DETECTION; PEROXIDASE-ACTIVITY; AMPLIFIED ANALYSIS; DNAZYME; ION; THROMBIN; APTAMERS; SENSOR; OLIGONUCLEOTIDE; NANOPARTICLES;
D O I
10.1039/b913036e
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel visual method for K+ detection is developed utilizing a K+-sensitive G-quadruplex DNA named PS5.M as the sensing element. In the absence of K+, PS5.M is in a random coil state and does not bind hemin. Upon addition of K+, PS5.M folds into the quadruplex structure stabilized by K+. Such quadruplex structure is able to bind hemin to form the hemin-G-quadruplex DNAzyme that catalyzes the H2O2-mediated oxidation of colorless 3,3',5,5'-tetramethylbenzidine to the blue product. Under the optimal conditions, the color change can be clearly observed with the naked eye in the concentration range from 2 to 1000 mM. Through this facile approach, K+ can be detected down to 2 mu M, with high selectivity against other cations. In view of its simplicity, sensitivity and specificity, our developed visual method for K+ detection would potentially be utilized for bioassays and K+-sensitive test paper.
引用
收藏
页码:71 / 75
页数:5
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