Thiol-functionalized gold surfaces as a strategy to induce order in membrane-bound enzyme immobilization

被引：23

作者：

Casero, E

Darder, M

Pariente, F

Lorenzo, E

Martín-Benito, J

Vázquez, L

机构：

[1] CSIC, Inst Ciencia Mat, Madrid 28049, Spain

[2] CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

[3] Univ Autonoma Madrid, Dept Quim Analit & Anal Instrumental, Madrid 28049, Spain

来源：

NANO LETTERS | 2002年 / 2卷 / 06期

关键词：

D O I：

10.1021/nl0255505

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have devised a strategy to induce order at the nanometer level in a membrane-bound enzyme layer, fructose dehydrogenase, on gold substrates. The procedure is based on an adequate choice of the chemical and morphological properties of the thiol, with respect to the protein structure, used as intermediate self-assembled layer. The nanometer order of the protein layer was assessed by means of atomic force microscopy operating in buffer solutions.

引用

页码：577 / 582

页数：6

共 31 条

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