Production of manganese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings.: Application to the decolorization of synthetic dyes

被引:23
作者
Couto, SR [1 ]
Domínguez, A [1 ]
Sanromán, A [1 ]
机构
[1] Univ Vigo, Dept Chem Engn, Vigo 36200, Spain
关键词
D O I
10.1007/BF02818701
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of manganese-dependent peroxidase (MnP) by Phanerochoete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 mukat/l, were obtained, respectively. The in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).
引用
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页码:417 / 421
页数:5
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