Chemiluminescence flow injection analysis of amoxycillin by a permanganate-based reaction

A new application of chemiluminescence for the determination of amoxycillin in flow injection mode is presented. The method is based upon the direct chemiluminescence reaction of amoxycillin with KMnO4 in acidic medium in presence of quinine sulfate. The chemiluminescence intensity was linearly related to the concentration of amoxycillin within 0.05-10 mug mL(-1) range with a detection limit (3sigma) of 0.02 mug mL(-1) and a sample measurement frequency of 90 samples h(-1). The relative standard deviation is 2.3% for 1.0 mug mL(-1) amoxycillin solution in 11 repeated measurements. The method was successfully applied to the analysis of amoxycillin in capsules.