Involvement of heat shock factor in regulating transcriptional activation of MDR1 gene in multidrug-resistant cells

被引：30

作者：

Kim, SH ^{[1
]}

Hur, WY ^{[1
]}

Kang, CD ^{[1
]}

Lim, YS ^{[1
]}

Kim, DW ^{[1
]}

Chung, BS ^{[1
]}

机构：

[1] CHANG WON UNIV,DEPT MICROBIOL,CHANG WON 641241,SOUTH KOREA

来源：

CANCER LETTERS | 1997年 / 115卷 / 01期

关键词：

HSF; MDR1; gene; heat shock protein; H-87; multidrug-resistant cells;

D O I：

10.1016/S0304-3835(97)04725-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The present study provides evidence that heat shock factor (HSF) may be involved in a transacting factor modulating multidrug resistance 1 (MDR1) gene. In conjunction with the presence of several heat shock elements (HSEs) in the 5' region of the MDR1 gene, we compared the level of HSF which binds to HSEs in multidrug-resistant P388/M and FM3A/M cells with that in their parental counterparts. Under unstressed condition, these multidrug-resistant cells showed constitutive HSF DNA-binding activity in the nucleus of the cells, whereas their parental counterparts did not show detectable HSF DNA-binding activity. We found that H-87, protein kinase A inhibitor, inhibited HSF DNA-binding activity in heat-shocked P388/M cells and also suppressed the levels of hsp90 and hsp70. These results demonstrated that HSF might be an important transcriptional regulator for inducing MDR1 gene, and modulation of HSF activity might be a useful potential target for reversing MDR. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：9 / 14

页数：6

共 20 条

[11] ACTIVATION OF HUMAN MULTIDRUG RESISTANCE-1 GENE PROMOTER IN RESPONSE TO HEAT-SHOCK STRESS [J].

MIYAZAKI, M ;

KOHNO, K ;

UCHIUMI, T ;

TANIMURA, H ;

MATSUO, K ;

NASU, M ;

KUWANO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 187 (02) :677-684

[12] COORDINATE CHANGES IN HEAT-SHOCK ELEMENT-BINDING ACTIVITY AND HSP70 GENE-TRANSCRIPTION RATES IN HUMAN-CELLS [J].

MOSSER, DD ;

THEODORAKIS, NG ;

MORIMOTO, RI .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (11) :4736-4744

[13]

OESTERREICH S, 1993, CANCER RES, V53, P4443

[14] MULTIDRUG RESISTANCE [J].

PASTAN, I ;

GOTTESMAN, MM .

ANNUAL REVIEW OF MEDICINE, 1991, 42 :277-286

[15] ACTIVATION OF HEAT-SHOCK GENE-TRANSCRIPTION BY HEAT-SHOCK FACTOR-I INVOLVES OLIGOMERIZATION, ACQUISITION OF DNA-BINDING ACTIVITY, AND NUCLEAR-LOCALIZATION AND CAN OCCUR IN THE ABSENCE OF STRESS [J].

SARGE, KD ;

MURPHY, SP ;

MORIMOTO, RI .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (03) :1392-1407

[16] DOWN-REGULATION OF MDR-1 EXPRESSION BY 8-CL-CAMP IN MULTIDRUG-RESISTANT MCF-7 HUMAN BREAST-CANCER CELLS [J].

SCALA, S ;

BUDILLON, A ;

ZHAN, ZR ;

CHOCHUNG, YS ;

JEFFERSON, J ;

TSOKOS, M ;

BATES, SE .

JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (02) :1026-1034

[17] HUMAN HEAT-SHOCK FACTOR-1 AND FACTOR-2 ARE DIFFERENTIALLY ACTIVATED AND CAN SYNERGISTICALLY INDUCE HSP70 GENE-TRANSCRIPTION [J].

SISTONEN, L ;

SARGE, KD ;

MORIMOTO, RI .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) :2087-2099

[18] YEAST HEAT-SHOCK FACTOR CONTAINS SEPARABLE TRANSIENT AND SUSTAINED RESPONSE TRANSCRIPTIONAL ACTIVATORS [J].

SORGER, PK .

CELL, 1990, 62 (04) :793-805

[19] INVOLVEMENT OF PROTEIN-KINASE IN ENVIRONMENTAL STRESS-INDUCED ACTIVATION OF HUMAN MULTIDRUG RESISTANCE-1 (MDR1) GENE PROMOTER [J].

UCHIUMI, T ;

KOHNO, K ;

TANIMURA, H ;

HIDAKA, K ;

ASAKUNO, K ;

ABE, H ;

UCHIDA, Y ;

KUWANO, M .

FEBS LETTERS, 1993, 326 (1-3) :11-16

[20] HUMAN HSP70 PROMOTER CONTAINS AT LEAST 2 DISTINCT REGULATORY DOMAINS [J].

WU, BJ ;

KINGSTON, RE ;

MORIMOTO, RI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (03) :629-633

← 1 2 →