Repeated electroacupuncture preconditioning attenuates matrix metalloproteinase-9 expression and activity after focal cerebral ischemia in rats
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作者:
Dong, Hui
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Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Dong, Hui
[1
]
Fan, Yan-Hong
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Fourth Mil Med Univ, Xijing Hosp, Dept Cardiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Fan, Yan-Hong
[2
]
Zhang, Wei
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Fourth Mil Med Univ, Dept Biochem & Mol Biol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Zhang, Wei
[3
]
Wang, Qiang
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Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Wang, Qiang
[1
]
Yang, Qian-Zi
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Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Yang, Qian-Zi
[1
]
Xiong, Li-Ze
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Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Xiong, Li-Ze
[1
]
机构:
[1] Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Xijing Hosp, Dept Cardiol, Xian 710032, Peoples R China
[3] Fourth Mil Med Univ, Dept Biochem & Mol Biol, Xian 710032, Peoples R China
Objective: This study investigates the effects of electroacupuncture (EA) preconditioning on blood-brain barrier (BBB) integrity and matrix metalloproteinase-9 (MMP-9) expression in subsequent ischemic hemisphere. Methods: Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. Animals were randomly divided into four groups: normal, sham-operated, MCAO and EA groups. In EA group, rats received electroacupuncture stimuli at the Baihui acupoint (GV 20) 30 minutes/day for 5 days. Twenty-four hours after last treatment, the MCAO was performed. The brain water content and BBB permeability were measured 24 hours after MCAO. MMP-9 expression and activity were measured at 6, 12 and 24 hours after MCAO. Results: The results showed that the brain water content of ischemic hemisphere was lower in EA group (81.45 +/- 1.09%) compared with MCAO group (83.98 +/- 1.30%; p<0.05). Similarly, the Evans blue content in EA group (4.90 +/- 1.77 mg/g) was lower compared with MCAO group (9.41 +/- 2.87 mg/g; p<0.05). The protein expression and enzyme activity of MMP-9 increased and reached maximum at 24 hours after reperfusion. However, the protein expression was lower in EA group at 12 and 24 hours after reperfusion (p<0.01, versus MCAO group), and enzyme activity was lower in EA group only at 24 hours (p<0.01, versus MCAO group). Discussion: EA preconditioning could attenuate brain edema and BBB disruption caused by subsequent cerebral ischemia. EA preconditioning could decrease MMP-9 expression and activity, which may be an important mechanism of cerebral ischemic tolerance. [Neurol Res 2009; 31: 853-858]
机构:Hong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R China
Siu, FKW
;
Lo, SCL
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机构:Hong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R China
Lo, SCL
;
Leung, MCP
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机构:
Hong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R China
机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Wang, Q
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Xiong, LZ
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Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Xiong, LZ
;
Chen, SY
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Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Chen, SY
;
Liu, YH
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机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Liu, YH
;
Zhu, XL
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机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
机构:Hong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R China
Siu, FKW
;
Lo, SCL
论文数: 0引用数: 0
h-index: 0
机构:Hong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R China
Lo, SCL
;
Leung, MCP
论文数: 0引用数: 0
h-index: 0
机构:
Hong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Rehabil Sci, Hong Kong, Hong Kong, Peoples R China
机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Wang, Q
;
Xiong, LZ
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Xiong, LZ
;
Chen, SY
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Chen, SY
;
Liu, YH
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h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
Liu, YH
;
Zhu, XL
论文数: 0引用数: 0
h-index: 0
机构:
Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R ChinaFourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China