Repeated electroacupuncture preconditioning attenuates matrix metalloproteinase-9 expression and activity after focal cerebral ischemia in rats

被引:58
作者
Dong, Hui [1 ]
Fan, Yan-Hong [2 ]
Zhang, Wei [3 ]
Wang, Qiang [1 ]
Yang, Qian-Zi [1 ]
Xiong, Li-Ze [1 ]
机构
[1] Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Xijing Hosp, Dept Cardiol, Xian 710032, Peoples R China
[3] Fourth Mil Med Univ, Dept Biochem & Mol Biol, Xian 710032, Peoples R China
关键词
Blood-brain barrier; cerebral ischemic tolerance; electroacupuncture preconditioning; matrix metalloproteinase-9; ARTERY OCCLUSION; MATRIX METALLOPROTEINASES; BRAIN; TOLERANCE; NEUROINFLAMMATION; RESVERATROL; REPERFUSION; INHIBITION; ADENOSINE; INJURY;
D O I
10.1179/174313209X393960
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Objective: This study investigates the effects of electroacupuncture (EA) preconditioning on blood-brain barrier (BBB) integrity and matrix metalloproteinase-9 (MMP-9) expression in subsequent ischemic hemisphere. Methods: Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. Animals were randomly divided into four groups: normal, sham-operated, MCAO and EA groups. In EA group, rats received electroacupuncture stimuli at the Baihui acupoint (GV 20) 30 minutes/day for 5 days. Twenty-four hours after last treatment, the MCAO was performed. The brain water content and BBB permeability were measured 24 hours after MCAO. MMP-9 expression and activity were measured at 6, 12 and 24 hours after MCAO. Results: The results showed that the brain water content of ischemic hemisphere was lower in EA group (81.45 +/- 1.09%) compared with MCAO group (83.98 +/- 1.30%; p<0.05). Similarly, the Evans blue content in EA group (4.90 +/- 1.77 mg/g) was lower compared with MCAO group (9.41 +/- 2.87 mg/g; p<0.05). The protein expression and enzyme activity of MMP-9 increased and reached maximum at 24 hours after reperfusion. However, the protein expression was lower in EA group at 12 and 24 hours after reperfusion (p<0.01, versus MCAO group), and enzyme activity was lower in EA group only at 24 hours (p<0.01, versus MCAO group). Discussion: EA preconditioning could attenuate brain edema and BBB disruption caused by subsequent cerebral ischemia. EA preconditioning could decrease MMP-9 expression and activity, which may be an important mechanism of cerebral ischemic tolerance. [Neurol Res 2009; 31: 853-858]
引用
收藏
页码:853 / 858
页数:6
相关论文
共 18 条
[11]   Bumetanide inhibition of the blood-brain barrier Na-L-Cl cotransporter reduces edema formation in the rat middle cerebral artery occlusion model of stroke [J].
O'Donnell, ME ;
Tran, L ;
Lam, TI ;
Liu, XB ;
Anderson, SE .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2004, 24 (09) :1046-1056
[12]   Matrix metalloproteinases in neuroinflammation [J].
Rosenberg, GA .
GLIA, 2002, 39 (03) :279-291
[13]   Electroacupuncture reduces the extent of lipid peroxidation by increasing superoxide dismutase and glutathione peroxidase activities in ischemic-reperfused rat brains [J].
Siu, FKW ;
Lo, SCL ;
Leung, MCP .
NEUROSCIENCE LETTERS, 2004, 354 (02) :158-162
[14]   Delayed treatment with an adenosine kinase inhibitor, GP683, attenuates infarct size in rats with temporary middle cerebral artery occlusion [J].
Tatlisumak, T ;
Takano, K ;
Carano, RAD ;
Miller, LP ;
Foster, AC ;
Fisher, M .
STROKE, 1998, 29 (09) :1952-1958
[15]   Rapid tolerance to focal cerebral ischemia in rats is induced by preconditioning with electroacupuncture: window of protection and the role of adenosine [J].
Wang, Q ;
Xiong, LZ ;
Chen, SY ;
Liu, YH ;
Zhu, XL .
NEUROSCIENCE LETTERS, 2005, 381 (1-2) :158-162
[16]   Induction of ischemic tolerance in rat cortical neurons by 3-nitropropionic acid: chemical preconditioning [J].
Weih, M ;
Bergk, A ;
Isaev, NK ;
Ruscher, K ;
Megow, D ;
Riepe, M ;
Meisel, A ;
Victorov, IV ;
Dirnagi, U .
NEUROSCIENCE LETTERS, 1999, 272 (03) :207-210
[17]  
Xiong L, 2003, CHINESE MED J-PEKING, V116, P108
[18]   Effects of ischemic preconditioning on blood-brain barrier permeability and MMP-9 expression of ischemic brain [J].
Zhang, FY ;
Chen, XC ;
Ren, HM ;
Bao, WM .
NEUROLOGICAL RESEARCH, 2006, 28 (01) :21-24