Retinal carotenoids can attenuate formation of A2E in the retinal pigment epithelium

被引：70

作者：

Bhosale, Prakash ^{[1
]}

Serban, Bogdan ^{[1
]}

Bernstein, Paul S. ^{[1
]}

机构：

[1] Univ Utah, Sch Med, Moran Eye Ctr, Dept Ophthalmol & Visual Sci, Salt Lake City, UT 84132 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2009年 / 483卷 / 02期

关键词：

Lutein; Zeaxanthin; Lipofuscin; A2E; Retinal pigment epithelium (RPE); Retina; Age-related macular degeneration (AMD); Photo-oxidation; Xanthophyll; Carotenoid; LIPOFUSCIN ACCUMULATION; MACULAR DEGENERATION; VITAMIN-A; MOUSE MODEL; LUTEIN; FLUOROPHORES; PHOTOOXIDATION; IDENTIFICATION; INVOLVEMENT; MECHANISMS;

D O I：

10.1016/j.abb.2008.09.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A2E, an important constituent of lipofuscin in human retinal pigment epithelium (RPE), is thought to mediate light-induced oxidative damage associated with aging and other ocular disorders. Ocular carotenoids in overlying retinal tissues were measured by HPLC and mass spectrometry and were correlated with levels of RPE A2E. We observed a statistically significant increase in total A2E levels in human RPE/choroid with age, and A2E levels in macular regions were approximately 1/3 lower than in peripheral retinal regions of the same size. There was a statistically significant inverse correlation between peripheral retina carotenoids and peripheral RPE/choroid A2E. Prospective carotenoid supplementation studies in Japanese quail demonstrated nearly complete inhibition of A2E formation and oxidation. These findings Support current recommendations to increase dietary intake of xanthophyll carotenoids in individuals at risk for macular degeneration and highlight a new potential mechanism for their Protective effects-inhibition of A2E formation and oxidation in the eye. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：175 / 181

页数：7

共 37 条

[1]

[Anonymous], 1993, Arch Ophthalmol, V111, P104

[2] Enhanced accumulation of A2E in individuals homozygous or heterozygous for mutations in BEST1 (VMD2) [J].

Bakall, B. ;

Radu, R. A. ;

Stanton, J. B. ;

Burke, J. M. ;

Mckay, B. S. ;

Wadelius, C. ;

Mullins, R. F. ;

Stone, E. M. ;

Travis, G. H. ;

Marmorstein, A. D. .

EXPERIMENTAL EYE RESEARCH, 2007, 85 (01) :34-43

[3] The role of oxidative stress in the pathogenesis of age-related macular degeneration [J].

Beatty, S ;

Koh, HH ;

Henson, D ;

Boulton, M .

SURVEY OF OPHTHALMOLOGY, 2000, 45 (02) :115-134

[4] Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients [J].

Bernstein, PS ;

Zhao, DY ;

Wintch, SW ;

Ermakov, IV ;

McClane, RW ;

Gellermann, W .

OPHTHALMOLOGY, 2002, 109 (10) :1780-1787

[5] Production of deuterated zeaxanthin by Flavobacterium multivorum and its detection by resonance Raman and mass spectrometric methods [J].

Bhosale, P ;

Teredesai, PV ;

Jin, LH ;

Ermakov, IV ;

Gellermann, W ;

Bernstein, PS .

BIOTECHNOLOGY LETTERS, 2005, 27 (21) :1719-1723

[6] Identification and metabolic transformations of carotenoids in ocular tissues of the japanese quail Coturnix japonica [J].

Bhosale, Prakash ;

Serban, Bogdan ;

Zhao, Da You ;

Bernstein, Paul S. .

BIOCHEMISTRY, 2007, 46 (31) :9050-9057

[7] Identification of 3-methoxyzeaxanthin as a novel age-related carotenoid metabolite in the human macula [J].

Bhosale, Prakash ;

Zhao, Da You ;

Serban, Bogdan ;

Bernstein, Paul S. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2007, 48 (04) :1435-1440

[8] HPLC measurement of ocular carotenoid levels in human donor eyes in the lutein supplementation era [J].

Bhosale, Prakash ;

Zhao, Da You ;

Bernstein, Paul S. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2007, 48 (02) :543-549

[9] Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods [J].

Bhosale, Prakash ;

Serban, Bogdan ;

Bernstein, Paul S. .

BIOTECHNOLOGY LETTERS, 2006, 28 (17) :1371-1375

[10]

Britton G., 1995, Carotenoids: Spectroscopy, V1B, P13

← 1 2 3 4 →