Preparation of chitosan-dopamine-multiwalled carbon nanotubes nanocomposite for electrocatalytic oxidation and sensitive electroanalysis of NADH

被引：77

作者：

Ge, Bin ^{[1
]}

Tan, Yueming ^{[1
]}

Xie, Qingji ^{[1
]}

Ma, Ming ^{[1
]}

Yao, Shouzhuo ^{[1
]}

机构：

[1] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ China, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 137卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Electrocatalytic oxidation of NADH; Multiwalled carbon nanotubes; Dopamine; Chitosan; Nanocomposite; NICOTINAMIDE ADENINE-DINUCLEOTIDE; LOW-POTENTIAL DETECTION; TOLUIDINE-BLUE-O; MODIFIED ELECTRODES; ELECTROCHEMICAL OXIDATION; METHYLENE-GREEN; PASTE ELECTRODES; GLUCOSE-OXIDASE; GOLD ELECTRODES; P-BENZOQUINONE;

D O I：

10.1016/j.snb.2009.01.030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel nanocomposite of glutaraldehyde crosslinked chitosan (CS)-dopamine (DA) composite with multiwalled carbon nanotubes (MWCNTs) was synthesized for low-potential and sensitive amperometric sensing of reduced nicotinamide adenine dinucleotide (NADH). The composite was characterized by FT-IR spectrophotometry. The nanocomposite modified Au electrode showed well-defined quinone/phenol redox peaks of the dopamine moieties and was able to effectively mediate the oxidation of NADH in pH 7.0 phosphate buffer, with an overpotential decrease by ca. 450 mV (vs. bare Au). The NADH electrochemical sensor showed a linear range of 0.1-600 mu M, a limit of detection of 12 nmol L-1, good antiinterferent ability, and long-term stability. It was also found that the use of acidified MWCNTs showed better performance than that of the nonacidified MWCNTs. Both CS and DA being biomaterials, the CS-DA/MWCNTs system should be of interest and potential for developing in vivo NAD(+) (the oxidized form of NADH)-dependent dehydrogenase enzymes based biosensors. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：547 / 554

页数：8

共 68 条

[41] Iron-mediated generation of the neurotoxin 6-hydroxydopamine quinone by reaction of fatty acid hydroperoxides with dopamine: A possible contributory mechanism for neuronal degeneration in Parkinson's disease [J].