Periodic signaling controlled by an oscillatory circuit that includes protein kinases ERK2 and PKA

被引:132
作者
Maeda, M
Lu, SJ
Shaulsky, G
Miyazaki, Y
Kuwayama, H
Tanaka, Y
Kuspa, A
Loomis, WF [1 ]
机构
[1] Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA
[2] Osaka Univ, Grad Sch Sci, Dept Biol, Osaka 5600043, Japan
[3] Baylor Coll Med, Dept Biochem & Mol Biol, Houston, TX 77030 USA
[4] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[5] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan
关键词
D O I
10.1126/science.1094647
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Self-regulating systems often use robust oscillatory circuits. One such system controls the chemotactic signaling mechanism of Dictyostelium, where pulses of adenosine 3', 5'-monophosphate (cAMP) are generated with a periodicity of 7 minutes. We have observed spontaneous oscillations in activation of the mitogen-activated protein (MAP) kinase ERK2 that occur in phase with peaks of cAMP, and we show that ERK2 modulates cAMP levels through the phosphodiesterase RegA. Computer modeling and simulations of the underlying circuit faithfully account for the ability of the cells to spontaneously generate periodic pulses during specific stages of development. Similar oscillatory processes may occur in cells of many different species.
引用
收藏
页码:875 / 878
页数:4
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