Egg activation at fertilization: Where it all begins

被引:276
作者
Runft, LL [1 ]
Jaffe, LA [1 ]
Mehlmann, LM [1 ]
机构
[1] Univ Connecticut, Ctr Hlth, Dept Physiol, Farmington, CT 06030 USA
关键词
fertilization; calcium; egg activation;
D O I
10.1006/dbio.2002.0600
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
A centrally important factor in initiating egg activation at fertilization is a rise in free Ca2+ in the egg cytosol. In echinoderm, ascidian, and vertebrate eggs, the Ca2+ rise occurs as a result of inositol trisphosphate-mediated release of Ca2+ from the endoplasmic reticulum. The release of Ca2+ at fertilization in echinoderm and ascidian eggs requires SH2 domain-mediated activation of a Src family kinase (SFK) and phospholipase C(PLC)gamma. Though some evidence indicates that a SFK and PLC may also function at fertilization in vertebrate eggs, SH2 domain-mediated activation of PLCgamma appears not to be required. Much work has focused on identifying factors from sperm that initiate egg activation at fertilization, either as a result of sperm-egg contact or sperm-egg fusion. Current evidence from studies of ascidian and mammalian fertilization favors a fusion-mediated mechanism; this is supported by experiments indicating that injection of sperm extracts into eggs causes Ca2+ release by the same pathway as fertilization. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:237 / 254
页数:18
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