RNA folding and unfolding

被引：85

作者：

Onoa, B

Tinoco, I

机构：

[1] DuPont Co Inc, Cent Res & Dev Expt Stn, Wilmington, DE 19880 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2004年 / 14卷 / 03期

关键词：

D O I：

10.1016/j.sbi.2004.04.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule studies of RNA folding and unfolding are providing impressive details of the intermediates that occur and their rates of interconversion. The folding and unfolding of RNA are controlled by varying the concentration of magnesium ions and measuring fluorescence energy transfer, or by applying force to the RNA and measuring the end-to-end distance. The hierarchical nature of RNA folding - first secondary structure, then tertiary structure - makes the process susceptible to analysis and prediction.

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收藏

页码：374 / 379

页数：6

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